Biocompatible bimetallic Au-Ni doped graphitic carbon nitride sheets: A novel peroxidase-mimicking artificial enzyme for rapid and highly sensitive colorimetric detection of glucose
Biocompatible bimetallic Au-Ni doped graphitic carbon nitride sheets: A novel peroxidase-mimicking artificial enzyme for rapid and highly sensitive colorimetric detection of glucose
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DOI：
10.1016/j.snb.2019.01.048
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发表时间：
2019-04-15
影响因子：
8.4
通讯作者：
Das, Manash R.
中科院分区：
文献类型：
--
作者：
Darabdhara, Gitashree;Bordoloi, Jijnasa;Das, Manash R.
我们在此报告了一种新型二维（2D）纳米复合材料传感器，可通过简单的比色检测技术检测葡萄糖。通过简单的化学还原技术，在石墨氮化碳（g-C3N4）片上装饰了尺寸约为11 nm的Au-Ni双金属纳米粒子。 Au-Ni/g-C3N4 纳米复合材料具有内在的过氧化物酶样活性，能够在 H2O2 存在下催化 3,3',5,5'-四甲基联苯胺 (TMB) 和 2,2'-azino-bis(3-乙基苯并噻唑啉-6-磺酸) (ABTS) 的氧化，并且表现出更高的底物亲和力 比生物辣根过氧化物酶以及其他报道的纳米酶。此外，关于基于双金属 g-C3N4 的纳米酶用于检测与人类生命相关的任何生物分子的文献报道也很少。类似过氧化物酶的活性与葡萄糖氧化酶 (GO(x)) 相结合，有助于开发一种简单、灵敏且特异的葡萄糖检测比色方法，检测限为 1.7 μM，线性检测范围为 0.5。 30 mu M。用于检测葡萄糖的比色法进一步成功地扩展到检测真实血清样品中的葡萄糖，其结果与标准医院方法相当。此外，纳米复合材料对人脐静脉内皮细胞系（HUVEC）无细胞毒性，这表明其具有高生物相容性和生物传感器应用的适用性。因此，我们的结果表明，我们基于 Au-Ni/g-C3N4 的方法是可靠、经济、可行且高效的。
We report herein, a novel two dimensional (2D) nanocomposite sensor towards detection of glucose via a simple colorimetric detection technique. The graphitic carbon nitride (g-C3N4) sheets are decorated with Au-Ni bimetallic nanoparticles with size similar to 11 nm by a simple chemical reduction technique. The Au-Ni/g-C3N4 nanocomposites is found to possess intrinsic peroxidase like activity capable of catalysing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) in presence of H2O2 and exhibits substrate affinity much higher than biological horseradish peroxidase as well as other reported nanozymes. Also, there is a scanty of literature reports on bimetallic g-C3N4 based nanozymes towards detection of any biomolecules related to human life. The peroxidase like activity combined with glucose oxidase (GO(x)) helped in developing a simple, sensitive and a specific colorimetric method for glucose detection with a detection limit of 1.7 mu M and a linear detection range between 0.5. 30 mu M. The colorimetric method for detection of glucose was further successfully extended towards detection of glucose in real serum samples with comparable results to standard hospital method. Additionally, the nanocomposites are non-cytotoxic towards human umbilical vein endothelial cell line (HUVEC) which demonstrates its high biocompatibility and suitability for biosensor applications. Our results thus demonstrate that our method based on Au-Ni/g-C3N4 is reliable, economical, feasible and highly efficient.