Differential effects of dietary copper deficiency and excess on lipid metabolism in yellow catfish Pelteobagrus fulvidraco
Differential effects of dietary copper deficiency and excess on lipid metabolism in yellow catfish Pelteobagrus fulvidraco
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DOI：
10.1016/j.cbpb.2015.02.004
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发表时间：
2015-06-01
影响因子：
2.2
通讯作者：
Hu, Wei
中科院分区：
文献类型：
--
作者：
Chen, Qi-Liang;Luo, Zhi;Hu, Wei
本研究旨在探讨饲料中铜的缺乏和过量对黄姑鱼幼鱼肝脏、肌肉和VAT中脂肪代谢的影响及其机制。为此，分别饲喂0.76(缺铜)、4.18(适量铜)和92.45(铜过量)mg铜kg(-1)饲料8周。铜充足组的WG和SGR显著高于铜缺乏组和铜过量组。在肝脏中，铜缺乏对铜含量、脂质含量、6PGD、G6PD和Fas的活性以及6PGD、G6PD、Fas、ACCα、PPARγ、LXR、HSL、PPARα和ATGL等多个被试基因的mRNA水平没有显著影响，但降低了脂质含量、Fas活性和6PGD、G6PD、Fas、ACCα、PPARγ、HSL和ATGL的mRNA水平。在肌肉中，日粮铜水平对脂肪含量、成脂酶活性以及大多数受试基因(包括6PGD、G6PD、Fas、SREBP-1、PPAR Gamma、HSL和LPL)的mRNA水平没有显著影响。饲料铜水平对VAT中铜含量、脂肪含量、Fas活性以及6PGD、G6PD、Fas、SREBP-1、LXR、PPARa和LPL的mRNA水平无显著影响。因此，组织间脂肪含量的变化可能与脂肪代谢相关的酶活性和基因表达有关。不同组织的酶活性和基因表达对饲料铜水平的响应模式不同，表明铜具有组织特异性的调节作用。(C)2015 Elsevier Inc.保留所有权利。
The present study was conducted to investigate the effects and mechanism of dietary Cu deficiency and excess on lipid metabolism in the liver, muscle and VAT of juvenile Pelteobagrus fulvidraco. To this end, yellow catfish were fed 0.76 (Cu deficiency), 4.18 (adequate Cu) and 92.45 (Cu excess) mg Cu kg(-1) diet, respectively, for 8 weeks.. WG and SGR in the adequate Cu group were significantly higher than those in Cu deficiency and excess groups. In liver, Cu deficiency showed no significant effect on Cu and lipid contents, the activities of 6PGD, G6PD and FAS, and the mRNA levels of many tested genes, including 6PGD, G6PD, FAS, ACC alpha, PPAR gamma, LXR, HSL, PPAR alpha and ATGL Cu excess induced Cu accumulation, reduced the lipid content, FAS activity as well as the mRNA levels of 6PGD, G6PD, FAS, ACC alpha, PPAR gamma, HSL and ATGL. In muscle, dietary Cu levels showed no significant effects on lipid content the activities of lipogenic enzymes and the mRNA levels of the most tested genes, including of 6PGD, G6PD, FAS, SREBP-1, PPAR gamma, HSL and LPL. In VAT, Cu and lipid contents, FAS activity, and the mRNA levels of 6PGD, G6PD, FAS, SREBP-1, LXR, PPARa and LPL were not significantly influenced by dietary Cu levels. Thus, the change of lipid contents among tissues could be related to the enzymatic activities and gene expression related to lipid metabolism. Different response patterns of enzymatic activities and gene expression in various tissues following dietary Cu levels indicated the tissue-specific regulatory effect by Cu. (C) 2015 Elsevier Inc All rights reserved.