Effect of feed composition on PHB production from methanol by HCDC of Methylobacterium extorquens (DSMZ 1340)
Effect of feed composition on PHB production from methanol by HCDC of Methylobacterium extorquens (DSMZ 1340)
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DOI：
10.1002/jctb.2145
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发表时间：
2009-08-01
影响因子：
3.4
通讯作者：
Heidarzadeh-Vazifekhoran, Ali
中科院分区：
文献类型：
--
作者：
Mokhtari-Hosseini, Zahra Beagom;Vasheghani-Farahani, Ebrahim;Heidarzadeh-Vazifekhoran, Ali
背景技术背景：聚对羟基丁酸酯（Poly-p-hydroxybutyrate，简称PHB）是由几种细菌产生的一种可生物降解的生物相容性化合物。不幸的是，由于生产成本高，其使用目前受到限制。克服这种限制的最常见方法之一是在高细胞密度培养（HCDC）中使用廉价的底物，如甲醇。采用优化的饲料配方进行发酵（639 g l（-1）甲醇，4 g l（-1）MgSO 4·7 H2O，41 mL L-1微量元素，5.6 g L-1 NaH 2 PO 4中心点H2O和24.3 g L-1 K2 HPO 4）和基于通过溶解氧（DO）检测底物限制的进料策略。35 h后，菌体干重（DCW）达到70 g L-1，此时菌体的生物量和PHB的生产率分别为2.8和0.98 g L-1 h（-1）。这些结果超过了文献中报道的由Methylobacterium species.CONCLUSION从甲醇生产PHB的Methylobacterium extorquens 1340在补料分批培养中的建议饲料组成和饲料策略导致高生物量和PHB生产率。它们可以用于由M.由于在生长阶段缺乏PHB积累，因此会产生extroquens。(C)2009年化学工业学会
BACKGROUND: Poly-p-hydroxybutyrate (PHB), produced by several species of bacteria, has attracted great attention as a biodegradable and biocompatible compound with similar properties to polypropylene. Unfortunately, its use is currently limited due to high production costs. One of the most common methods for overcoming this constraint is the use of inexpensive substrates, like methanol, in high cell density cultivations (HCDC).RESULTS: Fermentation was carried out with optimized feed composition (639 g l(-1) methanol, 4 g l(-1) MgSO4.7H2O, 41 mL L-1 trace elements, 5.6 g L-1 NaH2PO4 center dot H2O and 24.3 g L-1 K2HPO4) and a feeding strategy based on the detection of substrate limitation by dissolved oxygen (DO). After 35 h, at which dry cell weight (DCW) reached a value of 70 g L-1, PHB production was stimulated, and biomass and PHB productivities of 2.8 and 0.98 g L-1 h(-1) were obtained, respectively. These results surpassed those reported in the literature for PHB production from methanol by Methylobacterium species.CONCLUSION: The proposed feed composition and feeding strategy for PHB production from methanol by Methylobacterium extorquens 1340 in fed-batch cultivation resulted in high biomass and PHB productivity. They can be implemented for recombinant bioproducts (proteins) produced by M. extorquens due to the lack of PHB accumulation in the growth phase. (C) 2009 Society of Chemical Industry