Adsorption-resistant acrylic copolymer for prototyping of microfluidic devices for proteins and peptides
Adsorption-resistant acrylic copolymer for prototyping of microfluidic devices for proteins and peptides
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DOI：
10.1021/ac0617621
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发表时间：
2007-03-01
影响因子：
7.4
通讯作者：
Lee, Milton L.
中科院分区：
文献类型：
--
作者：
Liu, Jikun;Sun, Xuefei;Lee, Milton L.
开发了一种聚（乙二醇）官能化的丙烯酸共聚物，用于制造抗蛋白质和肽吸附的微流控器件。用该共聚物制作了具有典型十字图案的平面微毛细管电泳（μ CE）装置，以方便样品的引入。与大多数用于制造聚合物微芯片的方法相比，在这项工作中报道的共聚物使用的基于光聚合的方法是软光刻类型，图案化和粘合可以在10分钟内完成。在成品微器件中，盖板和图案化的基板通过强共价键结合在一起。此外，由于共聚物对吸附的抗性，可以在没有表面改性的情况下使用制造的微流体装置来分离蛋白质和肽。使用这些新的聚合物μ CE微芯片完成了异硫氰酸荧光素标记的蛋白质和肽样品的分离。使用3.5 cm分离通道，在不到40 s的时间内获得高达4.7 x 10（4）个板的分离效率，产生对称的肽和蛋白质峰。
A poly(ethylene glycol)-functionalized acrylic copolymer was developed for fabrication of microfluidic devices that are resistant to protein and peptide adsorption. Planar microcapillary electrophoresis (mu CE) devices were fabricated from this copolymer with the typical cross pattern to facilitate sample introduction. In contrast to most methods used to fabricate polymeric microchips, the photopolymerization-based method used with the copolymer reported in this work was of the soft lithography type, and both patterning and bonding could be completed within 10 min. In a finished microdevice, the cover plate and patterned substrate were bonded together through strong covalent bonds. Additionally, because of the resistance of the copolymer to adsorption, fabricated microfluidic devices could be used without surface modification to separate proteins and peptides. Separations of fluorescein isothiocyanate-labeled protein and peptide samples were accomplished using these new polymeric mu CE microchips. Separation efficiencies as high as 4.7 x 10(4) plates were obtained in less than 40 s with a 3.5-cm separation channel, yielding peptide and protein peaks that were symmetrical.