DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes
DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes
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DOI：
10.1016/j.molcel.2012.06.028
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发表时间：
2012-07-27
期刊：
影响因子：
16
通讯作者：
Teichmann, Sarah A.
中科院分区：
文献类型：
--
作者：
Charoensawan, Varodom;Janga, Sarath Chandra;Teichmann, Sarah A.
转录因子（TF）和组蛋白八聚体是两类丰富的DNA结合蛋白，它们协调细胞中的转录程序。对单个TF的详细研究表明，TF与核小体封闭的DNA序列结合并诱导核小体破坏/重新定位，而最近的全球研究表明，这不是所有TF使用的唯一机制。我们已经分析了在何种程度上的内在的DNA结合偏好的TF和组蛋白发挥作用，在确定核小体占有率，除了非内在因素，如染色质重塑的酶活性。芽殖酵母中的大多数TF具有与核小体组蛋白重叠的内在序列偏好。具有与组蛋白高度相关的内在DNA结合特性的TF倾向于与基因活化相关，并且可能与组蛋白竞争结合基因组DNA。与此相一致，我们表明，激活剂诱导更多的核小体破坏转录激活比阻遏。
Transcription factors (TFs) and histone octamers are two abundant classes of DNA binding proteins that coordinate the transcriptional program in cells. Detailed studies of individual TFs have shown that TFs bind to nucleosome-occluded DNA sequences and induce nucleosome disruption/repositioning, while recent global studies suggest this is not the only mechanism used by all TFs. We have analyzed to what extent the intrinsic DNA binding preferences of TFs and histones play a role in determining nucleosome occupancy, in addition to nonintrinsic factors such as the enzymatic activity of chromatin remodelers. The majority of TFs in budding yeast have an intrinsic sequence preference overlapping with nucleosomal histones. TFs with intrinsic DNA binding properties highly correlated with those of histones tend to be associated with gene activation and might compete with histones to bind to genomic DNA. Consistent with this, we show that activators induce more nucleosome disruption upon transcriptional activation than repressors.