Nanobubble Reactivity: Evaluating Hydroxyl Radical Generation (or Lack Thereof) under Ambient Conditions.
Nanobubble Reactivity: Evaluating Hydroxyl Radical Generation (or Lack Thereof) under Ambient Conditions.
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DOI：
10.1021/acsestengg.3c00124
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发表时间：
2023-06
影响因子：
7.1
通讯作者：
S. Chae;Min Sik Kim;Jae-Hong Kim;J. Fortner
中科院分区：
文献类型：
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作者：
S. Chae;Min Sik Kim;Jae-Hong Kim;J. Fortner
纳米气泡（NB）产生活性氧（ROS），特别是羟基自由基（·OH）一直是有争议的。在这项工作中，我们广泛地表征了溶液中的NB，重点是ROS的产生（作为·OH），通过多种方法，包括降解·OH-特异性目标化合物，电子顺磁共振（EPR）和基于荧光的指示剂。与以前的研究相比，生成的NB表现出一致的物理特性（大小，表面电位和浓度）。对于所描述的条件（被认为是高O2 NB浓度），在NB存在下（超过24 h）未观察到苯甲酸（BA）（一种经过充分研究的·OH清除剂）的降解，也未检测到·OH的EPR信号。当使用·OH，氨基苯基荧光素（APF）的荧光探针时，测量到阳性荧光响应，我们对结果提供了另一种解释。气/液界面表征表明NB的表面富含质子，并且能够诱导APF的酸催化水解，这导致假（阳性）荧光响应。鉴于这些负面结果，我们得出结论，NB诱导的·OH生成是最小的，如果有的话，对于评估的条件。
Nanobubble (NB) generation of reactive oxygen species (ROS), especially hydroxyl radical (·OH), has been controversial. In this work, we extensively characterize NBs in solution, with a focus on ROS generation (as ·OH), through a number of methods including degradation of ·OH-specific target compounds, electron paramagnetic resonance (EPR), and a fluorescence-based indicator. Generated NBs exhibit consistent physical characteristics (size, surface potential, and concentration) when compared with previous studies. For conditions described, which are considered as high O2 NB concentrations, no degradation of benzoic acid (BA), a well-studied ·OH scavenger, was observed in the presence of NBs (over 24 h) and no EPR signal for ·OH was detected. While a positive fluorescence response was measured when using a fluorescence probe for ·OH, aminophenyl fluorescein (APF), we provide an alternate explanation for the result. Gas/liquid interfacial characterization indicates that the surface of a NB is proton-rich and capable of inducing acid-catalyzed hydrolysis of APF, which results in a false (positive) fluorescence response. Given these negative results, we conclude that NB-induced ·OH generation is minimal, if at all, for conditions evaluated.