Solvent-free production of 1,3-diglyceride of CLA: Strategy consideration and protocol design
Solvent-free production of 1,3-diglyceride of CLA: Strategy consideration and protocol design
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DOI：
10.1016/j.foodchem.2005.11.011
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Sun, Yan
中科院分区：
文献类型：
--
作者：
Guo, Zheng;Sun, Yan
使用 Novozym 435 作为生物催化剂，以共轭亚油酸 (CLA) 作为模型脂肪酸，通过酶法生产均质的多不饱和脂肪酸 (PUFA) 1,3-二甘油酯。研究了三种不同的操作模式，即真空磁力搅拌、真空驱动的 N-2 鼓泡和分子筛孵育，以找到一种有效的酶生产方案。对传质影响的研究表明，传质限制的发生很大程度上取决于操作模式。事实证明，真空驱动的 N-2 鼓泡能够消除传质阻力，为多相反应系统创造有效的相互作用，并实现有效的除水和更快的反应速率。因此，真空驱动的 N2 搅拌被认为是生产具有工业价值的纯 PUFA 1,3-二甘油酯的测试策略中的最佳选择，因为它可以抑制 1,3-二甘油酯向 1,2-二甘油酯的酰基迁移，从而获得更高的目标产品产率、更高的生产率和更低的杂质含量。当5 mmol甘油与10-12 mmol CLA在45-55℃和小于10 mbar的压力下孵育约3小时，酶加载量为40-70 g l(-1)时，可以获得92-96%的1,3-dCLG。在操作参数中，温度和反应时间对酰基迁移和 1,3-甘油二酯的产率具有深远的影响。此外，该酶在优化条件下在此方案中表现出优异的操作稳定性（连续10批反应后观察到酶活性损失很少），表明该技术具有工业应用的潜力。 (c) 2005 Elsevier Ltd. 保留所有权利。
Enzymatic production of a homogeneous 1,3-diglyceride of polyunsaturated fatty acids (PUFAs) was carried out using Novozym 435 as biocatalyst and conjugated linoleic acid (CLA) as a model fatty acid. Three different operation modes, namely, magnetic stirring under vacuum, vacuum-driven N-2 bubbling and incubation with molecular sieves, were examined to find an efficient protocol for the enzymatic production. Studies on the effects of mass transfer showed that the occurrence of mass transfer limitation was strongly dependent on the operational modes. Vacuum-driven N-2 bubbling proved to be capable of eliminating mass transfer resistance, creating effective interaction for a multiple-phase reaction system and yielding an efficient water removal and a faster reaction rate. Hence, vacuum-driven N2 stirring was considered as the best choice among the tested strategies for the production of pure 1,3-diglyceride of PUFAs with industrial interests, because it gave a higher yield of the desired product, higher productivity and lower impurity content due to its suppression of the acylmigration of 1,3-diglyceride to 1,2-diglyceride. The yield of 92-96% 1,3-dCLG could be obtained when 5 mmol of glycerol were incubated with 10-12 mmol CLA for about 3 h at 45-55 degrees C and a pressure less than 10 mbar, with enzyme loading of 40-70 g l(-1). Among the operational parameters, temperature and reaction time were found to have profound effects on the acylmigration and yield of 1,3-diglyceride. Moreover, the enzyme showed excellent operational stability in this protocol under the optimized conditions (little activity loss of enzyme was observed after 10 consecutive batch reactions), indicating the potential of this technology for industrial application. (c) 2005 Elsevier Ltd. All rights reserved.