Shape-Recoverable Hyaluronic Acid–Waterborne Polyurethane Hybrid Cryogel Accelerates Hemostasis and Wound Healing
Shape-Recoverable Hyaluronic Acid–Waterborne Polyurethane Hybrid Cryogel Accelerates Hemostasis and Wound Healing
复制标题
可恢复形状的透明质酸 — 水性聚氨酯混合冷冻凝胶可加速止血和伤口愈合
DOI：
10.1021/acsami.2c01310
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hong Tan
中科院分区：
文献类型：
--
作者：
Min Wang;Juanjuan Hu;Yangcen Ou;Xueling He;Yanjun Wang;Chenyu Zou;Yanlin Jiang;Feng Luo;Dan Lu;Zhen Li;Jiehua Li;Hong Tan
促进快速止血并且在愈合伤口方面高效的伤口敷料是迫切需要的，以满足全球临床需求的增加。本文中，二酰肼改性的水性生物可降解聚氨酯乳液（PU-ADH）和氧化透明质酸（OHA）在−20 °C下通过腙键合自主交联以形成杂化透明质酸-聚氨酯（HA-PU）冻凝胶。通过其由聚集的PU-ADH颗粒和长链OHA构成的特定大孔结构（约220 μm），干燥的冻凝胶可以具有显著压缩的体积（其原始体积的1/7）并具有稳定的固定性，并且可以通过在几分钟内吸收水或血液分别膨胀至其干重的约22倍和16倍而迅速膨胀。这种瞬时形状恢复能力有利于微创手术中的快速止血。此外，该冷冻凝胶比纱布优越上级，具有优异的生物相容性，并通过激活内源性凝血系统快速凝血（约2分钟）。相比之下，在室温下制备了与HA-PU冻凝胶具有相同组分的可注射HA-PU水凝胶，其表现出良好的自修复性能。大鼠肝脏止血模型和大鼠皮肤缺损模型的体内评价表明，冷冻凝胶在快速止血方面具有巨大的潜力和优越的上级伤口愈合能力，减少免疫炎症，促进血管生成和毛囊再生。因此，这项工作提出了一种通用的方法，通过合成的聚合物乳液和天然聚合物之间的自主交联来构建可生物降解的杂化冻凝胶。混合冷冻凝胶在作为高性能伤口敷料的应用中表现出巨大的潜力。
Wound dressings that promote quick hemostasis and are highly efficient in healing wounds are urgently needed to meet the increase in clinical demands worldwide. Herein, a dihydrazide-modified waterborne biodegradable polyurethane emulsion (PU-ADH) and oxidized hyaluronic acid (OHA) were autonomously cross-linked to form a hybrid hyaluronic acid–polyurethane (HA-PU) cryogel by hydrazone bonding at −20 °C. Through its specific macroporous structure (which is approximately 220 μm) constructed by aggregated PU-ADH particles and long-chain OHA, a dried cryogel can have a dramatically compressed volume (1/7 of its original volume) with stable fixation, and it can swell rapidly by absorbing water or blood to approximately 22 and 16 times its dried weight, respectively, in a few minutes. This instantaneous shape-recovering ability favors fast hemostasis in minimally invasive surgery. Moreover, this cryogel is superior to gauze, has excellent biocompatibility, and quickly coagulates blood (in approximately 2 min) by activating the endogenous coagulation system. Comparably, an injectable HA-PU hydrogel with the same components as the HA-PU cryogel was prepared at room temperature, and it exhibited good self-healing properties. Anin vivoevaluation of a rat liver hemostasis model and rat skin defect model revealed that the cryogel in fast hemostasis has great potential and superior wound-healing abilities, decreases immune inflammation, and promotes the regeneration of angiogenesis and hair follicles. Consequently, this work proposes a versatile method for constructing biodegradable hybrid cryogels via autonomous cross-linking between synthesized polymer emulsions and natural polymers. The hybrid cryogels demonstrated great potential for applications as high-performance wound dressings.