Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light-Harvesting Proteins.
Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light-Harvesting Proteins.
复制标题
将天然植物类囊体组装成二维微阵列图案的脂质膜模型作为评估光捕获蛋白质的组织和光物理学的平台。
DOI：
10.1002/smll.202006608
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Meredith SA
中科院分区：
文献类型：
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作者：
Meredith SA
在绿色植物中发现的天然光合作用“类囊体”膜包含一个大的光收集（LH）蛋白复合物网络。这种光合作用机制的重新排列，侧向在被称为“颗粒”的堆叠膜内，改变了蛋白质之间的相互作用，导致系统内能量平衡的变化。通过在玻璃表面的二维微阵列模式中将类囊体膜和合成脂质连接到由聚合脂质组成的模板中，可以制备一个实验可访问的模型系统，从而可以详细研究这些复杂的相互作用。本文使用该系统在微观和纳米尺度上询问LH蛋白的行为，并评估该模型的有效性。荧光寿命成像和原子力显微镜相结合揭示了天然类囊体膜和杂交膜在光物理状态和横向组织方面的差异，LH蛋白掺入发育中的杂交膜的机制，以及系统的纳米级结构。每个畜栏内产生的模型系统是一个高质量的支持脂质双分子层，包含横向移动LH蛋白。在杂交膜和蛋白脂质体中评估光合活性，揭示了通常用于测试光系统II电子传递活性的光化学分析实际上可能产生假阳性结果。
Natural photosynthetic “thylakoid” membranes found in green plants contain a large network of light‐harvesting (LH) protein complexes. Rearrangement of this photosynthetic machinery, laterally within stacked membranes called “grana”, alters protein–protein interactions leading to changes in the energy balance within the system. Preparation of an experimentally accessible model system that allows the detailed investigation of these complex interactions can be achieved by interfacing thylakoid membranes and synthetic lipids into a template comprised of polymerized lipids in a 2D microarray pattern on glass surfaces. This paper uses this system to interrogate the behavior of LH proteins at the micro‐ and nanoscale and assesses the efficacy of this model. A combination of fluorescence lifetime imaging and atomic force microscopy reveals the differences in photophysical state and lateral organization between native thylakoid and hybrid membranes, the mechanism of LH protein incorporation into the developing hybrid membranes, and the nanoscale structure of the system. The resulting model system within each corral is a high‐quality supported lipid bilayer that incorporates laterally mobile LH proteins. Photosynthetic activity is assessed in the hybrid membranes versus proteoliposomes, revealing that commonly used photochemical assays to test the electron transfer activity of photosystem II may actually produce false‐positive results.