Ultra-sensitive aptasensor based on a GQD nanocomposite for detection of hepatitis C virus core antigen
Ultra-sensitive aptasensor based on a GQD nanocomposite for detection of hepatitis C virus core antigen
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DOI：
10.1016/j.ab.2017.07.016
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发表时间：
2017-10-01
影响因子：
2.9
通讯作者：
Azadbakht, Azadeh
中科院分区：
文献类型：
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作者：
Ghanbari, Kazhal;Roushani, Mahmoud;Azadbakht, Azadeh
在本研究中，通过使用适体邻近结合测定策略，描述了一种新型电化学适体传感器，用于超灵敏检测丙型肝炎病毒（HCV）核心抗原。固定表面是通过用石墨烯量子点（GQD）修饰玻碳电极（GCE）来制备的。通过π-π堆积相互作用，GQD被引入作为一种新型且合适的适体底物，GQD中丰富的亲水边缘和疏水平面增强了电极表面上的适体吸收。在化学改性过程的每个阶段进行电化学阻抗谱（EIS）、循环伏安法（CV）和微分脉冲伏安法（DPV），以确认所产生的表面变化。 EIS技术作为HCV核心抗原测量的有效替代检测系统，检测限为3.3 pg mL(-1)，具有10-70 pg mL(-1)和70-400 pg mL(-1)两个线性浓度范围，而且所构建的适配体传感器可以准确检测人血清样本中的HCV核心抗原浓度。这种适体传感器为HCV核心抗原检测开辟了一条快速、选择性和灵敏的途径，并为临床诊断的潜在应用提供了有前景的策略。 (C) 2017 Elsevier Inc. 保留所有权利。
In the present study, by using the aptamer proximity binding assay strategy, a novel electrochemical aptasensor is described for ultrasensitive detection of hepatitis C virus (HCV) core antigen. The immobilization surface is prepared by the modification of a glassy carbon electrode (GCE) with a graphene quantum dots (GQD). GQD were introduced as a novel and suitable substrate for aptamers through pi-pi stacking interactions, the richness of hydrophilic edges as well as hydrophobic plane in GQD which enhances the aptamer absorption on the electrode surface. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were performed at each stage of the chemical modification process to confirm the resulting surface changes. EIS technique was used as an efficient alternative detection system for HCV core antigen measurement with detection limit 3.3 pg mL(-1) and two linear concentration range 10-70 pg mL(-1) and 70-400 pg mL(-1) Moreover, the fabricated aptasensor could accurately detect HCV core antigen concentration in human serum samples. Such an aptasensor opens a rapid, selective and sensitive route for HCV core antigen detection and provides a promising strategy for potential applications in clinical diagnostics. (C) 2017 Elsevier Inc. All rights reserved.