Fructose intake during gestation and lactation differentially affects the expression of hippocampal neurosteroidogenic enzymes in rat offspring
Fructose intake during gestation and lactation differentially affects the expression of hippocampal neurosteroidogenic enzymes in rat offspring
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DOI：
10.1080/07435800.2016.1182186
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发表时间：
2017-01
影响因子：
2.1
通讯作者：
Genki Mizuno;Eiji Munetsuna;Hiroya Yamada;Y. Ando;Mirai Yamazaki;Yuri Murase;Kanako Kondo;Hiroaki Ishikawa;R. Teradaira;Koji Suzuki;Koji Ohashi
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文献类型：
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作者：
Genki Mizuno;Eiji Munetsuna;Hiroya Yamada;Y. Ando;Mirai Yamazaki;Yuri Murase;Kanako Kondo;Hiroaki Ishikawa;R. Teradaira;Koji Suzuki;Koji Ohashi
神经甾体是脑内由胆固醇重新合成的甾体激素，刺激海马神经元保护和突触形成等功能。在此之前，我们研究了母体果糖对神经类固醇生成酶的转录调控的影响。我们发现类固醇生成急性调节蛋白（星星）、外周苯二氮卓受体（PBR）、细胞色素P450（11β）、11β-羟基类固醇脱氢酶（HSD）和17β-HSD的mRNA表达水平发生了改变。然而，我们无法确定母体果糖摄入量是否在妊娠期或哺乳期发挥作用，因为母鼠在这两个时期都喂食果糖溶液。因此，在这项研究中，我们分析了在妊娠期或哺乳期喂食果糖的母鼠的后代的体重。无论是妊娠期还是哺乳期，母体果糖摄入量均不影响星星、P450（17α）、11β-HSD-2和17β-HSD-1的mRNA水平。PBR表达下调，即使大鼠仅在哺乳期摄入果糖，而妊娠期摄入果糖倾向于激活P450（11β）-2的表达。我们发现，母亲在妊娠期和哺乳期果糖摄入量差异影响海马神经类固醇生成酶的表达在后代。
ABSTRACT Neurosteroids, steroidal hormones synthesized de novo from cholesterol within the brain, stimulate hippocampal functions such as neuron protection and synapse formation. Previously, we examined the effect of maternal fructose on the transcriptional regulation of neurosteroidogenic enzymes. We found that the mRNA expression level of the steroidogenic acute regulatory protein (StAR), peripheral benzodiazepine receptor (PBR), cytochrome P450(11β), 11β-hydroxysteroid dehydrogenase (HSD), and 17β-HSD was altered. However, we could not determine whether maternal fructose intake played a role in the gestation or lactation period because the dam rats were fed fructose solution during both periods. Thus, in this study, we analyzed the hippocampi of the offspring of dams fed fructose during the gestation or lactation period. Maternal fructose consumption during either the gestation or lactation period did not affect the mRNA levels of StAR, P450(17α), 11β-HSD-2, and 17β-HSD-1. PBR expression was down-regulated, even when rats consumed fructose during the lactation period only, while fructose consumption during gestation tended to activate the expression of P450(11β)-2. We found that maternal fructose intake during gestation and lactation differentially affected the expression of hippocampal neurosteroidogenic enzymes in the offspring.