Developmental and genetic regulation of human surfactant protein B in vivo.
Developmental and genetic regulation of human surfactant protein B in vivo.
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作者：
Hamvas A;Heins HB;Guttentag SH;Wegner DJ;Trusgnich MA;Bennet KW;Yang P;Carlson CS;An P;Cole FS
Genetic and developmental disruption of surfactant protein B (SP-B) expression causes neonatal respiratory distress syndrome (RDS). Objectives: To assess developmental and genetic regulation of SP-B expression in vivo. To evaluate in vivo developmental regulation of SP-B, we used immunoblotting to compare frequency of detection of mature and pro-SP-B peptides in developmentally distinct cohorts: 24 amniotic fluid samples, unfractionated tracheal aspirates from 101 infants ≥34 weeks’ gestation with (75) and without (26) neonatal RDS, and 6 nonsmoking adults. To examine genetic regulation, we used univariate and logistic regression analyses to detect associations between common SP-B (SFTPB) genotypes and SP-B peptides in the neonatal RDS cohort. We found pro-SP-B peptides in 24/24 amniotic fluid samples and in 100/101 tracheal aspirates from newborn infants but none in bronchoalveolar lavage from normal adults (0/6) (p < 0.001). We detected an association (p = 0.0011) between pro-SP-B peptides (Mr 40 and 42 kDa) and genotype of a nonsynonymous single nucleotide polymorphism at genomic position 1580 that regulates amino-terminus glycosylation. Pro-SP-B peptides are more common in developmentally less mature humans. Association of genotype at genomic position 1580 with pro-SP-B peptides (Mr 40 and 42 kDa) suggests genetic regulation of amino terminus glycosylation in vivo.
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DOI：
10.1152/ajplung.00407.2001
发表时间：
2002-08-01
影响因子：
4.9
作者：
Kramer, BW;Kramer, S;Jobe, AH
通讯作者：
Jobe, AH
DOI：
10.1165/rcmb.2003-0262oc
发表时间：
2004-04-01
影响因子：
6.4
作者：
Brasch, F;Johnen, G;Ochs, M
通讯作者：
Ochs, M
影响因子：
3.6
作者：
Merrill, JD;Ballard, RA;Ballard, PL
通讯作者：
Ballard, PL
影响因子：
3.2
作者：
STAHLMAN, MT;GRAY, ME;WHITSETT, JA
通讯作者：
WHITSETT, JA
影响因子：
4.8
作者：
Korimilli, A;Gonzales, LW;Guttentag, SH
通讯作者：
Guttentag, SH