Dependance of sulfite reduction on a crystallized ferredoxin from Desulfovibrio gigas.
Dependance of sulfite reduction on a crystallized ferredoxin from Desulfovibrio gigas.
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亚硫酸盐还原对来自 Desulfovibrio gigas 的结晶铁氧还蛋白的依赖性。
DOI：
10.1016/0006-291x(66)90519-5
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发表时间：
1966
影响因子：
3.1
通讯作者：
N. Dragoni
中科院分区：
文献类型：
--
作者：
J. Gall;N. Dragoni
从脱硫脱硫弧菌中分离出铁氧还蛋白已被Tagawa等人简短地提及。（1962）和Akagi（1965）提出了一些证据，表明由产黑梭菌还原亚硫酸盐需要铁氧还蛋白。它也已被证明是一个颗粒级分，从D。如果将一些可溶性蛋白质添加到制剂中，gigas能够还原亚硫酸盐。这种可溶性蛋白质可以被分离成两个蛋白质级分的处理与DEAE纤维素。酸性蛋白质部分被认为含有铁氧还蛋白（Peck，1966）。这些数据强烈支持铁氧还蛋白对D还原亚硫酸盐的要求。千兆。本文研究了从D. gigas及其在亚硫酸盐还原中的作用。
The isolation from Desulfovibrio desulfuricans of a ferredoxin has been shortly mentionned by Tagawa et al.(1962), and Akagi (1965) has presented some evidence suggesting that reduction of sulfite by Clostridium nigrificans requires ferredoxin. It has also been shown that a particulate fraction from D. gigas was able to reduce sulfite if some soluble proteins were added to the preparation. This soluble protein could be separated into two protein fractions by treatment with DEAE cellulose. The acidic protein fraction was thought to contain ferredoxin (Peck, 1966). Such data were strongly in favour of a ferredoxin requirement for the reduction of sulfite by D. gigas. In the present paper, the purification of a ferredoxin from D. gigas and its role in sulfite reduction are reported.