Age-related liquefaction of the human vitreous body: LM and TEM evaluation of the role of proteoglycans and collagen
Age-related liquefaction of the human vitreous body: LM and TEM evaluation of the role of proteoglycans and collagen
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DOI：
10.1167/iovs.02-0588
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Hooymans, JMM
中科院分区：
文献类型：
--
作者：
Los, LI;van der Worp, RJ;Hooymans, JMM
目的.通过光学显微镜和电子显微镜评价与年龄相关的人类玻璃体液化的形态学方面，为今后针对这种现象的发病机制进行研究提供基础。该研究的重点是纤维胶原和蛋白多糖（PGs）的变化。通过光学（LM）和透射电子显微镜（TEM）检查了13只成年人供体眼（年龄21-80岁）的玻璃体内液化间隙及其周围基质区域的形态学。通过使用标准对比方法使胶原纤维可视化。PG用铜分光光度计（CB）进行特异性染色。来自老年供体的眼睛比来自年轻供体的眼睛包含更大的空间。基质与空间之间的转换是突然的或渐进的。在所有标本的过渡区，胶原纤维的数量逐渐减少，并在较小程度上的PG观察。此外，还发现胶原纤维的断裂和PG分子在这些断裂周围的聚集，在这些区域内没有观察到细胞或其碎片。这是第一个在光镜和电镜水平上评价玻璃体液化的研究。胶原纤维分解成更小的碎片似乎是至关重要的发病机制与年龄有关的液化。人体玻璃体诱导玻璃体纤维碎裂的机制尚未阐明。从所有标本中的细胞和细胞修复的情况下，暂时得出结论，细胞外过程参与。（Invest Ophthalmol维斯科学。2003;44：2828-2833）DOI：10.1167/ iovs.02-0588。
PURPOSE. To evaluate morphologic aspects of age-related liquefaction of the human vitreous body by fight and electron microscopy to provide a basis from which future studies directed at the pathogenesis of this phenomenon can be undertaken. The study focuses on changes in fibrillar Collagen and proteoglycans; (PGs).METHODS. Morphologic aspects of intravitreal liquefied spaces and matrix areas surrounding them were examined in 13 adult human donor eyes (aged 21-80 years) by light (LM) and transmission electron microscopy (TEM). Collagen fibrils were visualized by using standard contrasting methods. PGs were specifically stained by cupromeronic blue (CB).RESULTS. Eyes from older donors contained larger spaces than eyes from younger ones. Transitions between matrix and spaces were abrupt or gradual. In transition areas of all specimens, a gradual decrease in the number of collagen fibers, and to a lesser extent of PGs was observed. In addition, a fragmentation of collagen fibers and an aggregation of PG-molecules around these fragments were found. Neither cells nor their fragments were observed in these areas.CONCLUSION. This is the first study to evaluate vitreous liquefaction at the fight and electron microscopic level. A breakdown of collagen fibrils into smaller fragments seems to be crucial to the pathogenesis of age-related liquefaction of. the human vitreous body. The mechanism inducing fragmentation of vitreous fibrils has yet to be elucidated. From the absence of cells and cellular reimnants in all specimens, it is tentatively concluded that an extracellular process is involved. (Invest Ophthalmol Vis Sci. 2003;44:2828-2833) DOI:10.1167/ iovs.02-0588.