Habituation-induced neural plasticity in the hippocampus and prefrontal cortex mediated by MMP-3.
Habituation-induced neural plasticity in the hippocampus and prefrontal cortex mediated by MMP-3.
复制标题
MMP-3 介导的海马和前额皮质中习惯诱导的神经可塑性。
DOI：
10.1016/j.bbr.2009.04.014
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发表时间：
2009
影响因子：
2.7
通讯作者：
Harding,JosephW
中科院分区：
文献类型：
--
作者：
Wright,JohnW;Meighan,PeterC;Brown,TravisE;Wiediger,RobertaV;Sorg,BarbaraA;Harding,JosephW
目前，摇头反应(HSR)习惯化被用来研究自发恢复和神经可塑性现象。对不同的大鼠进行连续的5次习服训练，训练间隔分别为2、24或72小时。测试结束后，采集大鼠海马区和前额叶皮质组织标本，检测神经可塑性标志物基质金属蛋白酶-3(MMP3：stromellysin-1)的表达。结果显示，到第5次，ISI后2小时组无自发恢复，72小时组接近完全自发恢复，而24小时组为中度恢复。缺血再灌注2、72h组大鼠海马区和前额叶皮质中基质金属蛋白酶-3表达增加，但24小时组未见表达。第二个实验利用7天的渗透泵在脑室内注入一种基质金属蛋白酶-3抑制剂，持续6天。然后在第7天使用2小时ISI方案对动物进行测试。给予基质金属蛋白酶-3抑制剂促进了动物的自然恢复，从而损害了动物适当适应的能力。伴随这一作用的是显著抑制海马区和前额叶皮质基质金属蛋白酶-3的表达。这些结果表明，海马区和前额叶皮质中基质金属蛋白酶-3的表达升高有助于这种最简单的学习形式，并可能是自发恢复的机制。
Head-shake response (HSR) habituation was presently used to investigate the phenomena of spontaneous recovery and neural plasticity. Independent groups of rats were presented with five consecutive habituation sessions separated by inter-session intervals (ISIs) of 2, 24 or 72h. At the conclusion of testing hippocampus and prefrontal cortex tissue samples were collected for determination of matrix metalloproteinase-3 (MMP-3:stromelysin-1) expression as a marker of neural plasticity. The results indicated that by the fifth session the 2h ISI group showed no spontaneous recovery, the 72h ISI group revealed nearly complete spontaneous recovery; while the 24h ISI group showed intermediate recovery. MMP-3 expression in the hippocampus and prefrontal cortex was elevated in the 2 and 72h ISI groups, but not in the 24h group. A second experiment utilized 7-day osmotic pumps to intracerebroventricularly infuse an MMP-3 inhibitor for 6 days. The animals were then tested on the seventh day using the 2h ISI protocol. Delivery of the MMP-3 inhibitor facilitated spontaneous recovery, thus compromising the animal's ability to appropriately habituate. This effect was accompanied by a significant inhibition of hippocampus and prefrontal cortex MMP-3 expression. These results suggest that elevations in hippocampus and prefrontal cortex MMP-3 expression contribute to this simplest form of learning and may be a mechanism underlying spontaneous recovery.