SODIUM AND POTASSIUM FLUX OF SEPARATED RENAL TUBULES.
SODIUM AND POTASSIUM FLUX OF SEPARATED RENAL TUBULES.
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分离肾小管的钠和钾通量。
DOI：
10.1152/ajplegacy.1964.206.3.483
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发表时间：
1964
期刊：
影响因子：
--
通讯作者：
Jack Orloff
中科院分区：
文献类型：
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作者：
Maurice B. Burg;E. F. Grollman;Jack Orloff
用家兔肾皮质分离的肾小管悬液测定了肾小管细胞的钠、钾通量。动力学分析表明，在肾小管中至少有两个钠室和两个钾室。Na和K交换的速率比肾皮质切片中的速率大得多，这与基于切片中细胞的几何排列考虑的预测一致。洋地黄样类固醇，已知抑制主动阳离子转运，降低了钠从主要钠室的流出速率常数，并减少了钾流入和钾流出速率常数。计算出的Na的主动流出量大大超过了K的流入量，表明主动Na和K转运不是以1：1的比例耦合的。
The Na and K fluxes of renal tubule cells were measured using suspensions of tubules separated from rabbit renal cortex. Kinetic analysis indicates that there are at least two Na compartments and two K compartments in the tubules. The rates at which Na and K exchanged were considerably greater than in renal cortical slices in accordance with predictions based on considerations of the geometrical arrangement of the cells in slices. Digitalis-like steroids, known to depress active cation transport, lowered the efflux rate constant for Na from the major Na compartment and diminished both K influx and the K efflux rate constants. The calculated active efflux of Na greatly exceeded the influx of K, indicating that active Na and K transport are not coupled in a 1:1 ratio.