IMMUNOCYTOCHEMICAL INVESTIGATIONS ON THE PRESENCE OF NEURON-SPECIFIC ANTIBODIES IN THE CSF OF PARKINSONS-DISEASE CASES
IMMUNOCYTOCHEMICAL INVESTIGATIONS ON THE PRESENCE OF NEURON-SPECIFIC ANTIBODIES IN THE CSF OF PARKINSONS-DISEASE CASES
复制标题
DOI：
10.1007/bf00973287
复制
发表时间：
1988-07-01
影响因子：
4.4
通讯作者：
DAHLSTROM, A
中科院分区：
文献类型：
--
作者：
MCRAEDEGUEURCE, A;ROSENGREN, L;DAHLSTROM, A
采用免疫细胞化学方法对7例帕金森病患者脑脊液及血清进行检测，观察戊二醛固定大鼠脑内是否存在识别da能细胞体的抗体。2例患者在黑质中观察到明显的da能神经元标记，通过抗da抗血清和6-OHDA诱导的变性识别，但也识别出腹侧中脑的其他神经元。其他患者呈弱阳性或阴性。血清对所有神经元进行非特异性标记。在一名患者中，IgG的亚类被调查，发现是IgG3（标记神经末梢）和IgG1-2，低亲和力型（识别核周围）。识别的表位尚未确定，但不太可能是da样的，因为阻断实验和elisa测试的结果为阴性。讨论了这些结果可能的临床意义。
The CSF and sera of 7 patients with Parkinson''s disease were investigated immunocytochemically, in order to see if antibodies were present which recognized DA-ergic cell bodies in glutaraldehyde fixed rat brain. In 2 patients a marked labeling of DA-ergic neurons in the substantia nigra was observed, identfied by anti-DA antiserum and by 6-OHDA induced degeneration, but also other neurons in the ventral mesencephalon were recognized. The other patients were weakly positive or negative. Sera gave unspecific labelling of all neurons. In one patient the sub-classes of IgG were investigated and found to be of IgG3 (labeling nerve terminals) and of IgG1-2, low affinity type (recognizing perikarya). The epitopes recognized have not been identified, but are unlikely to be DA-like, since blocking experiments and ELISA-tests gave negative results. The possible clinical importance of the results are discussed.