ALPHA-AMANITIN-RESISTANT D-MELANOGASTER WITH AN ALTERED RNA POLYMERASE-II
ALPHA-AMANITIN-RESISTANT D-MELANOGASTER WITH AN ALTERED RNA POLYMERASE-II
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DOI：
10.1016/0092-8674(79)90116-8
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
COULTER, DE
中科院分区：
文献类型：
--
作者：
GREENLEAF, AL;BORSETT, LM;COULTER, DE
在EMS（甲磺酸乙酯）诱变后，恢复了在对野生型致死的α-鹅膏蕈素浓度下生长的黑腹果蝇突变体。该突变体可能代表了鹅膏蕈碱抗性真核生物的第一个例子。突变体 (AmaC4) 的鹅膏菌素抗性是由于其 DNA 依赖性 RNA 聚合酶 II 的改变，无论是在细胞核、部分分级提取物中还是作为高度纯化的酶进行测试，其对鹅膏菌素抑制的敏感性比野生型聚合酶 II 低约 250 倍。而野生型酶活性则被抑制50%2.1倍。 10-8 M α-鹅膏蕈碱，抑制 50% 的 AmaC4 RNA 聚合酶 II 活性需要 5.6 倍的毒素浓度。 10-6 M。导致 AmaC4 鹅膏菌素抗性的突变位于靠近朱红色基因座的 X 染色体上。
A mutant of D. melanogaster that grows at concentrations of .alpha.-amanitin lethal to wild-type was recovered following EMS (ethylmethanesulfonate) mutagenesis. This mutant may represent the 1st example of an amanitin-resistant eukaryotic organism. The amanitin resistance of the mutant (AmaC4) is due to an alteration in its DNA-dependent RNA polymerase II, which is approximately 250 times less sensitive to inhibition by amanitin than the wild-type polymerase II whether tested in nuclei, in partially-fractionated extracts or as a highly purified enzyme. While the wild-type enzyme activity is inhibited 50% by 2.1 .times. 10-8 M .alpha.-amanitin, inhibition of 50% of the AmaC4 RNA polymerase II activity requires a toxin concentration of 5.6 .times. 10-6 M. The mutation responsible for the amanitin resistance of AmaC4 is on the X chromosome near the vermillion locus.