Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin
Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin
复制标题
用于靶向递送阿霉素的叶酸共轭介孔二氧化硅功能化氮化硼纳米球
DOI：
10.1016/j.msec.2018.11.063
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发表时间：
2019-03-01
影响因子：
7.9
通讯作者：
Gao, Xiao-Dong
中科院分区：
文献类型：
--
作者：
Feng, Shini;Zhang, Huijie;Gao, Xiao-Dong
氮化硼（BN）纳米材料的生物医学应用近来引起了人们的广泛关注。BN纳米球（BNNS）可以安全地将抗癌药物导入肿瘤细胞，是一种潜在的肿瘤治疗纳米载体。但其在生理环境中分散性差、载药量低等缺点严重限制了其进一步的应用。在此，我们开发了一种新的药物传递系统的基础上叶酸共轭介孔二氧化硅（MS）功能化的BNNS（BNMS-FA）。MS改性后BNNS的分散性和载药能力得到显著提高。BNMS-FA复合物在100 μ g/mL的浓度下是无毒的，并且可以通过叶酸受体介导的内吞作用被HeLa和MCF-7细胞特异性内化。阿霉素（DOX）通过π-π堆积和氢键作用高效负载于BNMS-FA复合物上，并在不同pH条件下表现出缓释模式。与游离DOX、BNNS/DOX和BNMS/DOX复合物相比，BNMS-FA/DOX复合物具有更好的上级药物内化和抗肿瘤作用，有望成为肿瘤靶向治疗的新材料。
Biomedical application of boron nitride (BN) nanomaterials has recently attracted considerable attentions. BN nanospheres (BNNS) could safely deliver anti-cancer drug into tumor cells, which makes them potential nano carrier for cancer therapy. However, the poor dispersity in physiological environments and low drug loading capacity severely limit their further applications. Herein, we developed a novel drug delivery system based on folate-conjugated mesoporous silica (MS)-functionalized BNNS (BNMS-FA). Dispersity and drug loading capacity of BNNS were highly improved by MS modification. BNMS-FA complexes were nontoxic up to a concentration of 100 mu g/mL, and could be specifically internalized by HeLa and MCF-7 cells via folate receptor-mediated endocytosis. Doxorubicin (DOX) could be loaded onto BNMS-FA complexes with high efficiency via pi-pi stacking and hydrogen bonding, and showed a sustained release pattern under different pH conditions. BNMS-FA/DOX complexes exhibited superior drug internalization and antitumor efficacy over free DOX, BNNS/DOX and BNMS/DOX complexes, which were considered promising for targeted cancer therapy.