EXPRESSION AND LOCALIZATION OF PROTEINS OF THE COMPLEMENT-SYSTEM IN HUMAN SKIN
EXPRESSION AND LOCALIZATION OF PROTEINS OF THE COMPLEMENT-SYSTEM IN HUMAN SKIN
复制标题
DOI：
10.1172/jci116080
复制
发表时间：
1992-11-01
影响因子：
15.9
通讯作者：
GIGLI, I
中科院分区：
文献类型：
--
作者：
DOVEZENSKI, N;BILLETTA, R;GIGLI, I
补体系统参与对外来抗原的免疫识别，其中许多抗原可通过物理损伤或经皮吸附穿透皮肤。在这项研究中，我们研究了存在的补体成分和补体调节蛋白在人类皮肤和培养的人角质形成细胞。免疫荧光研究表明，C3，因子B，衰变加速因子，C3 B受体（CR1），和C3 d受体（CR2），分布在表皮细胞以及培养的角质形成细胞。角质形成细胞上清液的免疫印迹分析显示存在分子量约为180 kD的C3。衰减加速因子的本地化，如先前报道的弹性纤维，此外，它被观察到在基底膜区。原位杂交研究表明，CR1和CR2 mRNA在人表皮表达。这些结果表明人表皮中存在能够产生旁路途径的初始C3转化酶的补体组分。补体调节蛋白的存在可以赋予角质形成细胞免疫功能，例如调节补体激活和C3调理颗粒的内吞作用。
The complement system participates in the immune recognition of foreign antigens, many of which may penetrate the skin by physical injury or transcutaneous adsorption. In this study, we examined the presence of complement components and complement regulatory proteins in the human skin and cultured human keratinocytes. Immunofluorescence studies showed C3, Factor B, decay accelerating factor, the C3b receptor (CR1), and C3d receptor (CR2), distributed among cells of the epidermis as well as on cultured keratinocytes. Immunoblot analysis of keratinocytes supernatants showed the presence of C3 with a molecular weight of approximately 180 kD. The decay accelerating factor was localized as previously reported on elastic fibers; additionally it was observed in the basement membrane zone. In situ hybridization studies suggest the expression of CR1 and CR2 mRNA in human epidermis. These results show the presence in the human epidermis of complement components that are capable of generating the initial C3 convertase of the alternative pathway. The presence of complement regulatory proteins could endow keratinocytes with immune functions such as the regulation of complement activation and endocytosis of C3 opsonized particles.