Inhibition of myogenic bHLH and MEF2 transcription factors by the bHLH protein twist
Inhibition of myogenic bHLH and MEF2 transcription factors by the bHLH protein twist
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DOI：
10.1126/science.272.5267.1476
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发表时间：
1996-06-07
期刊：
影响因子：
56.9
通讯作者：
Lassar, AB
中科院分区：
文献类型：
--
作者：
Spicer, DB;Rhee, J;Lassar, AB
生肌碱性螺旋-环-螺旋（bHLH）和MEF 2转录因子在发育体节的肌节中表达，并协同激活骨骼肌基因表达。bHLH蛋白Twist在整个上皮体节中表达，随后从肌节中排除。异位表达的小鼠Twist（Mtwist）通过阻断MyoD与DNA的结合、滴定E蛋白和抑制MEF 2的反式激活来抑制肌生成。为了抑制MEF 2，Mtwist需要与E蛋白和完整的碱性结构域和羧基末端异二聚化。因此，Mtwist抑制两个家族的肌原性调节剂，并可能调节肌节的形成时间或空间。
The myogenic basic helix-loop-helix (bHLH) and MEF2 transcription factors are expressed in the myotome of developing somites and cooperatively activate skeletal muscle gene expression. The bHLH protein Twist is expressed throughout the epithelial somite and is subsequently excluded from the myotome. Ectopically expressed mouse Twist (Mtwist) was shown to inhibit myogenesis by blocking DNA binding by MyoD, by titrating E proteins, and by inhibiting trans-activation by MEF2. For inhibition of MEF2, Mtwist required heterodimerization with E proteins and an intact basic domain and carboxyl-terminus. Thus, Mtwist inhibits both families of myogenic regulators and may regulate myotome formation temporally or spatially.