Difference in B cell activation between dermatomyositis and polymyositis: analysis of the expression of RP105 on peripheral blood B cells
Difference in B cell activation between dermatomyositis and polymyositis: analysis of the expression of RP105 on peripheral blood B cells
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DOI：
10.1136/ard.60.12.1137
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发表时间：
2001-12-01
影响因子：
27.4
通讯作者：
Nagasawa, K
中科院分区：
文献类型：
--
作者：
Kikuchi, Y;Koarada, S;Nagasawa, K
背景-已有研究表明，一种新的B细胞表面蛋白RP105在激活的人类B细胞中丢失。目的：以RP105为标记物，探讨皮肌炎(DM)和多发性肌炎(PM)患者外周血中RP105阴性B细胞活化的差异。方法：采用流式细胞术分析7例皮肌炎(DM)和11例多发性肌炎(PM)患者外周血中RP105阴性B细胞的数量。结果：PM患者外周血中RP105阴性B细胞比例较低(5.8(SD 2.4)%)。相比之下，所有糖尿病患者的RP105阴性B细胞亚群均增加(33.0(6.9)%)。1例DM和活动期间质性肺炎患者的支气管肺泡灌洗液中含有大量RP105阴性的B细胞。结论：RP105阴性的B细胞的增殖是DM的一个标志，DM和PM的B细胞活化在病理上可能不同
Background-It has previously been shown that RP105, a new B cell surface protein, is lost in activated human B cells. Objective-To investigate whether there is a difference in B cell activation between patients with dermatomyositis (DM) and those with polymyositis (PM) using RP105 as a marker.Methods-The population of RP105 negative B cells (activated B cells) in the peripheral blood mononuclear cells of seven patients with dermatomyositis (DM) and 11 with polymyositis (PM) was analysed by flow cytometry.Results-The percentage of RP105 negative B cells in the peripheral blood of patients with PM was low (5.8 (SD 2.4)%), similar to that of normal subjects. In contrast, all patients with DM showed increased RP105 negative B cell populations (33.0 (6.9)%). Bronchoalveolar lavage fluid from a patient with DM and active interstitial pneumonitis contained a large number of RP105 negative B cells.Conclusion-These findings suggest that the expansion of RP105 negative B cells is a hallmark of DM, and that B cell activation in DM may be pathogenetically different from that in PM.