Melissa officinalis Extract Inhibits Attachment of Herpes Simplex Virus in vitro
Melissa officinalis Extract Inhibits Attachment of Herpes Simplex Virus in vitro
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DOI：
10.1159/000335590
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发表时间：
2012-01-01
期刊：
影响因子：
3.3
通讯作者：
Schnitzler, Paul
中科院分区：
文献类型：
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作者：
Astani, Akram;Reichling, Juergen;Schnitzler, Paul
背景资料：由于单纯疱疹病毒（HSV）可能对常用的抗病毒药物产生耐药性，药用植物的提取物和精油作为抗疱疹剂的新型药物越来越受到关注。研究方法：本文研究了香蜂草水提物和酚类提取物化合物咖啡酸、对香豆酸和迷迭香酸的体外抗单纯疱疹病毒Ⅰ型（HSV-1）活性。结果如下：当将药物加入HSV-1感染的细胞中时，通过空斑减少测定和病毒蛋白ICP 0表达分析确定没有观察到抗病毒效果。然而，蜂花属植物提取物即使在1.5 μ g/ml的非常低的浓度下也表现出对HSV-1的高杀病毒活性，而酚类化合物仅在高100倍的浓度下才获得类似的结果。除杀病毒活性外，蜂花提取物和迷迭香酸以剂量依赖性方式抑制HSV-1与宿主细胞的附着。这些结果表明迷迭香酸是蜂花提取物抗病毒活性的主要贡献者。但875蜂花提取物对HSV的选择性指数优于单一成分的选择性指数，为上级。结论：蜂花提取物具有低毒、杀病毒作用，并能影响HSV-1对宿主细胞的粘附。版权所有（C）2012 S. Karger AG，巴塞尔
Background: Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs for antiherpetic agents, since the herpes simplex virus (HSV) might develop resistance to commonly used antiviral drugs. Methods: An aqueous extract of Melissa officinalis as well as phenolic extract compounds, i.e. caffeic acid, p-counnaric acid and rosmarinic acid were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. Results: When drugs were added to HSV-1-infected cells, no antiviral effect was observed as determined by plaque reduction assay and analysis of expression of viral protein ICP0. However, the Melissa extract demonstrated a high virucidal activity against HSV-1, even at very low concentrations of 1.5 mu g/ml, whereas similar results for phenolic compounds were only achieved at 100 times higher concentrations. Besides the virucidal activity, the Melissa extract and rosnnarinic acid inhibited HSV-1 attachment to host cells in a dose-dependent manner. These results indicate that rosmarinic acid was the main contributor to the antiviral activity of Melissa extract. However, the selectivity index of Melissa extract of 875 against HSV is superior to the selectivity indices of single constituents. Conclusion: Melissa extract exhibits low toxicity, is virucidal and affects HSV-1 attachment to host cells in vitro. Copyright (C) 2012 S. Karger AG, Basel