Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter
Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter
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DOI：
10.1039/b209333b
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发表时间：
2003-01-01
期刊：
影响因子：
6.1
通讯作者：
Telleman, P
中科院分区：
文献类型：
--
作者：
Wolff, A;Perch-Nielsen, IR;Telleman, P
完整的分析系统集成在芯片上是微加工器件的最大潜力之一。在这项研究中，我们提出了一种新的压力驱动的微制造荧光激活细胞分选芯片与先进的功能集成。使用该分选仪，荧光乳胶珠从鸡红细胞中分选出来，在12000个细胞s（-1）的样品通量下实现了大量富集。作为分选芯片的一部分，我们开发了一种单片集成的单步同轴流动化合物，用于流式细胞术和细胞分选中样品的流体动力学聚焦。该结构简单，易于微加工和与其他微流控组件集成。我们在芯片上设计了一个集成腔室，用于保存和培养分选的细胞。通过集成该腔室，消除了在细胞处理过程中丢失细胞的风险。此外，我们还开发了用于细胞检测的集成光学器件。我们的新设计有助于建立一个完全集成的微细胞分选和分析系统的持续努力。
The integration of complete analyses systems "on chip" is one of the great potentials of microfabricated devices. In this study we present a new pressure-driven microfabricated fluorescent-activated cell sorter chip with advanced functional integration. Using this sorter, fluorescent latex beads are sorted from chicken red blood cells, achieving substantial enrichments at a sample throughput of 12000 cells s(-1). As a part of the sorter chip, we have developed a monolithically integrated single step coaxial flow compound for hydrodynamic focusing of samples in flow cytometry and cell sorting. The structure is simple, and can easily be microfabricated and integrated with other microfluidic components. We have designed an integrated chamber on the chip for holding and culturing of the sorted cells. By integrating this chamber, the risk of losing cells during cell handling processes is eliminated. Furthermore, we have also developed integrated optics for cell detection. Our new design contributes to the ongoing efforts for building a fully integrated micro cell sorting and analysing system.