RNA EDITING AT A SPLICING SITE OF NADH DEHYDROGENASE SUBUNIT-IV GENE TRANSCRIPT IN WHEAT MITOCHONDRIA
RNA EDITING AT A SPLICING SITE OF NADH DEHYDROGENASE SUBUNIT-IV GENE TRANSCRIPT IN WHEAT MITOCHONDRIA
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DOI：
10.1016/0014-5793(89)81620-5
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发表时间：
1989-11-20
期刊：
影响因子：
3.5
通讯作者：
GRIENENBERGER, JM
中科院分区：
文献类型：
--
作者：
LAMATTINA, L;WEIL, JH;GRIENENBERGER, JM
以小麦线粒体总mtRNA为模板，将小麦线粒体NADH脱氢酶亚基IV(NAD4)的编码基因序列与逆转录获得的cDNA序列进行比较，发现在剪接转录本的外显子2-外显子3连接处存在一个非基因组序列编码的尿苷残基。这个U产生了一个非编码的CUG亮氨酸密码子，这是维持阅读框架所必需的，正如各种物种中NAD4蛋白的氨基酸序列保守所表明的那样。U的添加或C到U的特定转录后转换可以解释这种现象。
Comparison between the sequence of the gene coding for the wheat mitochondrial NADH dehydrogenase subunit IV (nad4) and the cDNA sequence obtained by reverse transcription, using total wheat mtRNA as template, has shown the presence of a uridine residue, not encoded by the genomic sequence, at the exon2‐exon3 junction of the spliced transcript. This U creates a non‐encoded CUG leucine codon which is essential for maintaining the reading frame, as shown by the conservation of the amino acid sequence of the NAD4 protein in various species. The addition of a U or the specific post‐transcriptional conversion of a C to a U could explain this phenomenon.