Nestin expression in neuroepithelial tumors
Nestin expression in neuroepithelial tumors
复制标题
DOI：
10.1016/j.neulet.2006.02.034
复制
发表时间：
2006-05-29
影响因子：
2.5
通讯作者：
Tamagno, Ilaria
中科院分区：
文献类型：
--
作者：
Schiffer, Davide;Manazza, Andrea;Tamagno, Ilaria
巢蛋白是神经细胞发生早期阶段的标志。通过免疫组织化学、免疫荧光、免疫印迹和共聚焦显微镜对50例神经上皮肿瘤（主要是不同恶性级别的胶质瘤）进行了研究，并与GEAP和Vimentin进行了比较。作为分化的早期标志，Nestin在弥漫性星形细胞瘤中几乎不表达，在间变性星形细胞瘤中表达可变，在胶质母细胞瘤中表达强烈且不规则。在少突胶质细胞瘤中阴性，在室管膜瘤中染色，在毛细胞星形细胞瘤中表达梯度。在胶质母细胞瘤中，Nestin的分布与GFAP和Vimentin的分布并不完全对应，共聚焦显微镜证实其在肿瘤细胞中的表达变化是互补的。因此，肿瘤细胞可以从神经细胞发生阶段衍生或向神经细胞发生阶段分化。假设它们可能与放射状胶质细胞有关，在胚胎形成过程中，这三种抗原依次表达。侵袭性或复发性胶质母细胞瘤的完全阴性细胞可能是突变积累后的恶性选择克隆或不表达抗原的早期干细胞。2006爱思唯尔爱尔兰有限公司版权所有。
Nestin is a marker of early stages of neurocytogenesis. It has been studied in 50 neuroepithelial tumors, mostly gliomas of different malignancy grades, by immunohistochemistry, immunofluorescence, immunoblotting, and confocal microscopy and compared with GEAP and Vimentin. As an early marker of differentiation, Nestin is almost not expressed in diffuse astrocytomas, variably expressed in anaplastic astrocytomas and strongly and irregularly expressed in glioblastomas. Negative in oligodendrogliomas, it stains ependymomas and shows a gradient of expression in pilocytic astrocytomas. In glioblastomas, Nestin distribution does not completely correspond to that of GFAP and Vimentin with which its expression varies in tumor cells in a complementary way, as confirmed by confocal microscopy. Tumor cells can thus either derive from or differentiate toward the neurocytogenetic stages. Hypothetically, they could be put in relation with radial glia where during embriogenesis the three antigens are successively expressed. Completely negative cells of invasive or recurrent glioblastomas may represent malignant selected clones after accumulation of mutations or early stem cells not expressing antigens. (c) 2006 Elsevier Ireland Ltd. All rights reserved.