Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells
Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells
复制标题
DOI：
10.1007/s11274-010-0495-3
复制
发表时间：
2011-03
影响因子：
4.1
通讯作者：
Zhihua Liu;Yanlei Ma;T. Shen;Hong-Qi Chen;Yu‐kun Zhou;P. Zhang;Ming Zhang;Zhao-xin Chu;H. Qin-H
中科院分区：
文献类型：
--
作者：
Zhihua Liu;Yanlei Ma;T. Shen;Hong-Qi Chen;Yu‐kun Zhou;P. Zhang;Ming Zhang;Zhao-xin Chu;H. Qin-H
植物乳杆菌可以通过调节人体免疫系统发挥额外的益生菌作用。然而，益生菌与免疫细胞受体之间的直接相互作用仍需进一步研究。鉴定树突状细胞在与L-1相互作用过程中的受体。车前草。树突状细胞用L。植物细胞和树突状细胞特异性细胞间黏附分子抓取非整合素(DC-SIGN)、Toll样受体(TLR)-2和TLR-4的抗体。用流式细胞仪检测未成熟树突状细胞的成熟、细胞因子的产生和T细胞的调节。L之间的粘附性。植物细胞和树突状细胞通过上调MHC-II、CD80和CD86的表达，经免疫印迹、流式细胞仪检测发现，植物细胞可以成熟树突状细胞。抗炎因子IL-10和IL-6表达上调，促炎因子IL-12p70表达上调。植物细胞可能与DC-SIGN相互作用，调节T细胞分化为产生IL-4的T细胞。L.的相互作用。其生物学效应可被EDTA和抗DC-SIGN抗体所阻断。L的影响。植物细胞能以不同比例与DC-SIGN结合，促进DC成熟，调节抗炎和促炎细胞因子的分泌，诱导产生IL-4的T细胞极化。
Lactobacillus plantarumcan exert additional probiotic effects via regulation of human immune system. However, the direct interaction between probiotics and the receptors of immune cells still needs to be further studied. To identify the receptor of dendritic cells during the interaction withL. plantarum. Dendritic cells were pretreated withL. plantarumand the antibody to dendritic cells specific intercellular adhesion molecule-grabbing nonintegrin (DC-SIGN), toll like receptor (TLR)-2 and TLR-4. The maturation of immature dendritic cells, cytokine production, and modulation of T cells were studied by flow cytometry. Adherence betweenL. plantarumand dendritic cells were studied by ELISA, flow cytometry, and Western blot.L. plantarumcould mature dendritic cells by up-regulating MHC-II and CD80 and CD86. Anti-inflammatory interlectin (IL)-10 and IL-6 was up-regulated and pro-inflammatory IL-12p70 was retro-regulated byL. plantarum.L. plantarummay interact with DC-SIGN and modulate of T to differentiate into IL-4 producing T cells. The interaction ofL. plantarumand DC-SIGN and the biological effects could be blocked by EDTA and antibody to DC-SIGN. Effects ofL. plantarumwere concentration-dependent.L. plantarumcould bind to DC-SIGN to improve DC maturation at different ratios, regulate the secretion of anti-inflammatory and pro-inflammatory cytokines, and induce the polarization of interlectin-4-producing T cells.