Molecular cloning and characterization of the porcine Ero1L and ERp44 genes: Potential roles in controlling energy metabolism
Molecular cloning and characterization of the porcine Ero1L and ERp44 genes: Potential roles in controlling energy metabolism
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猪 Ero1L 和 ERp44 基因的分子克隆和表征：在控制能量代谢中的潜在作用。
DOI：
10.1016/j.ygcen.2011.05.014
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Yang, Zaiqing
中科院分区：
文献类型：
--
作者：
Long, Qinqiang;Zhu, Xiaoyue;Yang, Zaiqing
二硫键的形成是分泌性蛋白质成熟和释放的关键步骤，由特定的内质网（ER）驻留酶控制。在这个过程中的一个重要因素是Ero（ER氧化还原），糖基化黄素酶与氧化蛋白质折叠，缺乏已知的ER保留基序紧密相关。ER驻留蛋白44 kDa（ERp44）是一种ER驻留蛋白，其介导ER中的ERo 1定位，并且还防止具有未配对半胱氨酸的未组装货物蛋白的分泌。这些蛋白质不仅是二硫键形成过程中的关键参与者，而且还在定性和定量水平上控制分泌途径。在这里，我们克隆了全长cDNA序列的猪Ero1L（1448 bp）和ERp44（1361 bp）基因。Ero1L基因包含16个外显子和15个内含子，长度约为150 kp，而ERp44基因包含12个外显子和11个内含子，长度超过140 kp，它们分别位于猪染色体1q21和1q29上。两个基因的组织分布分析显示，在脂肪组织中极高的表达，和拓扑结构的基因树表明不同物种之间的高度保守。我们研究了Ero1L和ERp44 5 '侧翼区域的转录因子结合位点，许多脂肪分化相关因子反映了与能量代谢的密切关系。(C)2011 Elsevier Inc. All rights reserved.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that is controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is Ero (ER oxidoreduction), a glycosylated flavoenzyme tightly associated with oxidative protein folding that lacks the known ER retention motifs. ER resident protein 44 kDa (ERp44) is an ER resident protein that mediates ERo1 localization in ER and also prevents the secretion of unassembled cargo proteins with unpaired cysteine. These proteins are not only the key participants in the disulfide-bond formation process, but they also control the secretory pathway on both qualitative and quantitative levels. Here, we cloned full-length cDNA sequences of the porcine Ero1L (1448 bp) and ERp44 (1361 bp) genes. Isolation and characterization of their genomic sequences revealed that Ero1L contains 16 exons and 15 introns almost 150 kp in length, whereas ERp44 contains 12 exons and 11 introns more than 140 kp in length, and they are located on porcine chromosome 1q21 and 1q29, respectively. Tissue distribution analysis of the two genes revealed extremely high expression in adipose tissue, and the topology of their phylogenic tree indicates a high degree of conservation among different species. We looked at transcription factors binding sites in the 5'-flanking regions of Ero1L and ERp44, and many adipose differentiations related factors reflect the tight relationship to energy metabolism. (C) 2011 Elsevier Inc. All rights reserved.