Developmental coupling of expression of the Igh-linked minor antigen H-40 to membrane immunoglobulin expression.
Developmental coupling of expression of the Igh-linked minor antigen H-40 to membrane immunoglobulin expression.
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Igh 连接次要抗原 H-40 的表达与膜免疫球蛋白表达的发育耦合。
DOI：
10.1097/00007890-198908000-00028
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发表时间：
1989
期刊：
影响因子：
6.2
通讯作者：
Forman,J
中科院分区：
文献类型：
--
作者：
Schreiber,KL;Webb,C;Tucker,P;Riblet,R;Forman,J
CB-20（Igh B）而不是BALB/c（Igh B）小鼠能够排斥BCL 1，BCL 1是BALB/c起源的自发性B细胞白血病。保护性免疫应答针对次要组织相容性（H）抗原H-40，其可被细胞毒性T淋巴细胞检测到，并在表面免疫球蛋白阳性肿瘤细胞（包括BCL 1）以及脂多糖活化的sIg+成淋巴细胞上表达。只有sIg+肿瘤细胞和淋巴母细胞表达H-40这一事实表明，sIg本身可能是参与CTL识别该抗原的一个成分。然而，这种可能性被排除，证明从靶细胞中去除sIg并不能阻止H-40特异性CTL识别。为了确定H-40的表达是否与膜IG的表达协同调节，我们确定了H-40− s IG −细胞在被诱导表达s IG时是否获得H-40。我们还将功能性c区和v区基因转染到sIg-淋巴瘤中，使其获得slg+表型。在这两种情况下，这样的slg+细胞都没有获得H-40。总之，这些数据表明H-40在代表与sIg一致的特定分化阶段的B细胞上表达。
CB-20 (Igh b) but not BALB/c (Igh b) mice are able to reject BCL 1, a spontaneous B cell leukemia of BALB/c origin. The protective immune response is directed to ward the minor histocompatibility (H) antigen, H-40, which can be detected by cytotoxic T lymphocytes and is expressed on surface (s) immunoglobulin positive tumor cells including BCL 1 as well as sIg+ lymphoblasts activated by lipopolysaccharide. The fact that only sIg+ tumor cells and lymphoblasts express H-40 suggests that sIg itself may be a component involved in CTL recognition of this antigen. However, this possibility was ruled out by demonstrating that removal of sIg from target cells did not prevent H-40-specific CTL recognition. To determine whether H-40 expression was coordinately regulated with that of membrane Ig, we determined whether H-40− sIg− cells acquire H-40 when induced to express sIg. We also transfected a functional c and v region gene into a sIg− lymphoma so that it acquired a slg+ phenotype. In neither case did such slg+ cells acquire H-40. Together, these data indicate that H-40 is expressed on B cells representing a particular stage of differentiation that is coincidental with slg.