Wistar rats: a forgotten model of age-related hearing loss.
Wistar rats: a forgotten model of age-related hearing loss.
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Wistar 大鼠：一种被遗忘的与年龄相关的听力损失模型。
DOI：
10.3389/fnagi.2014.00029
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发表时间：
2014
影响因子：
4.8
通讯作者：
Juiz JM
中科院分区：
文献类型：
--
作者：
Alvarado JC;Fuentes-Santamaría V;Gabaldón-Ull MC;Blanco JL;Juiz JM
听觉相关性听力损失（ARHL）是衰老中最常见的感觉障碍之一，是重要的社会经济后果的来源。了解患有这种残疾的患者的中枢听觉通路中发生的病理反应对于改善其诊断和治疗至关重要。因此，本研究的目的是表征年龄相关的修改听觉脑干反应（ABR），并确定这些功能性反应是否可能伴随着兴奋和抑制之间的不平衡，在耳蜗核的Wistar大鼠。为此，在不同频率的ABR记录和囊泡谷氨酸转运体1（VGLUT 1）和囊泡GABA转运体（VGAT）的腹侧耳蜗核（VCN）进行了免疫组织化学在年轻，中年和老年雄性Wistar大鼠。结果表明，随着年龄的增长，大鼠ABR各波的听阈显著增高，波幅显著降低，波幅增加。此外，与年轻大鼠相比，老年大鼠VCN中的VGLUT 1和VGAT免疫染色减少。因此，观察到的年龄相关的听觉诱发反应的幅度下降可能部分是由于兴奋性功能标志物的减少;同时，兴奋性和抑制性标志物的伴随减少可能反映了ARLH动物模型中常见的中枢改变。总之，这些发现突出了Wistar大鼠作为研究ARHL的优良模型的适用性。
Age-related hearing loss (ARHL) is one of the most frequent sensory impairments in senescence and is a source of important socio-economic consequences. Understanding the pathological responses that occur in the central auditory pathway of patients who suffer from this disability is vital to improve its diagnosis and treatment. Therefore, the goal of this study was to characterize age-related modifications in auditory brainstem responses (ABR) and to determine whether these functional responses might be accompanied by an imbalance between excitation and inhibition in the cochlear nucleus of Wistar rats. To do so, ABR recordings at different frequencies and immunohistochemistry for the vesicular glutamate transporter 1 (VGLUT1) and the vesicular GABA transporter (VGAT) in the ventral cochlear nucleus (VCN) were performed in young, middle-aged and old male Wistar rats. The results demonstrate that there was a significant increase in the auditory thresholds, a significant decrease in the amplitudes and an increase in the latencies of the ABR waves as the age of the rat increased. Additionally, there were decreases in VGLUT1 and VGAT immunostaining in the VCN of older rats compared to younger rats. Therefore, the observed age-related decline in the magnitude of auditory evoked responses might be due in part to a reduction in markers of excitatory function; meanwhile, the concomitant reduction in both excitatory and inhibitory markers might reflect a common central alteration in animal models of ARLH. Together, these findings highlight the suitability of the Wistar rat as an excellent model to study ARHL.