Ingestion of yogurt containing Lactobacillus acidophilus and Bifidobacterium to potentiate immunoglobulin A responses to cholera toxin in mice
Ingestion of yogurt containing Lactobacillus acidophilus and Bifidobacterium to potentiate immunoglobulin A responses to cholera toxin in mice
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DOI：
10.3168/jds.s0022-0302(99)75281-1
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发表时间：
1999-04-01
影响因子：
3.5
通讯作者：
Pestka, JJ
中科院分区：
文献类型：
--
作者：
Tejada-Simon, MV;Lee, JH;Pestka, JJ
据报道，乳酸菌有益于预防和治疗某些形式的腹泻和相关疾病。为了确定这些影响是否可能涉及直接刺激胃肠道免疫反应，我们给予酸奶，试图增强粘膜和全身抗体对口服免疫原，霍乱毒素。酸奶是用含有不同种类和菌株的乳酸菌的发酵剂制造的。用这些酸奶喂养小鼠3周，在此期间用10 μ g霍乱毒素口服免疫小鼠两次。在第0天和第21天采集血液，每周采集粪粒。口服霍乱毒素免疫的小鼠通过产生特异性肠和血清免疫球蛋白（IG）A抗霍乱毒素来应答。伊加同种型的抗体反应显着增加小鼠喂养酸奶含有传统的酸奶细菌保加利亚乳杆菌和嗜热链球菌补充嗜酸乳杆菌，两歧双歧杆菌，和双歧杆菌的启动器。用只含有传统酸奶细菌的发酵剂生产的酸奶比对照组的脱脂奶粉产生的伊加抗霍乱毒素少。虽然也观察到血清中IgG抗霍乱毒素的强烈反应，但各组之间的反应没有差异。因此，管理酸奶补充L，嗜酸乳杆菌和双歧杆菌属。增强的粘膜和全身伊加对霍乱毒素免疫原的应答。
Lactic acid bacteria have been reported to have benefits for the prevention and treatment of some forms of diarrhea and related conditions. To determine whether these effects might involve direct stimulation of the gastrointestinal immune response, we administered yogurt to try to enhance mucosal and systemic antibodies against an orally presented immunogen, cholera toxin. Yogurts were manufactured with starter cultures containing different species and strains of lactic acid bacteria. Mice were fed these yogurts for 3 wk, during which they were also orally immunized twice with 10 mu g of cholera toxin. Blood was collected on d 0 and 21, and fecal pellets were collected weekly. Mice that were immunized orally with cholera toxin responded by producing specific intestinal and serum immunoglobulin (Ig)A anti-cholera toxin. Antibody responses of the IgA isotype were significantly increased in mice fed yogurts made with starters containing the conventional yogurt bacteria Lactobacillus bulgaricus and Streptococcus thermophilus supplemented with Lactobacillus acidophilus, Bifidobacterium bifidum, and Bifidobacterium infantis. Yogurt that was manufactured with starters containing only conventional yogurt bacteria produced less IgA anti-cholera toxin than did the control group fed nonfat dry milk. Although strong responses were also observed for IgG anti-cholera toxin in serum, the responses did not differ among groups. Thus, administration of yogurt supplemented with L, acidophilus and Bifidobacterium spp. enhanced mucosal and systemic IgA responses to the cholera toxin immunogen.