Development of a Novel Antiadhesive Material, Alginate Flakes, Ex Vivo and In Vivo
Development of a Novel Antiadhesive Material, Alginate Flakes, Ex Vivo and In Vivo
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DOI：
10.1007/s00595-010-4410-1
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发表时间：
2011-07-01
期刊：
影响因子：
2.5
通讯作者：
Hagiwara, Akeo
中科院分区：
文献类型：
--
作者：
Hirasaki, Yoshinori;Fukunaga, Masaki;Hagiwara, Akeo
目的.术后腹腔内粘连有时会导致严重的并发症。本研究的目的是比较我们新开发的抗粘连材料，藻酸盐薄片，最常用的组合的透明质酸和羧甲基纤维素膜的功效。海藻酸钠形成凝胶、粉末或薄片。在离体研究中，将这些不同的藻酸盐形式附着到猪皮上，并比较它们在盐水中的抗溶解性和附着稳定性。在体内研究中，使用大鼠手术粘连模型来研究藻酸盐的性质，并在手术后第14天对大鼠实施安乐死。使用粘连评分系统评价抗粘连材料的有效性，并对用抗粘连材料治疗的部位进行组织学检查。在藻酸盐组中，藻酸盐薄片在离体抗溶解性和附着稳定性以及在体内抗粘附功效方面是优越的。粘附评分与藻酸盐片和纤维素膜组几乎相同。我们开发了一种藻酸盐薄片材料，并证明了其体外和体内的抗粘附作用。这是第一次报道使用这种片状材料的研究，这种材料具有独特的特性，因为它可以通过在压缩空气中喷涂来应用。因此，藻酸盐薄片在腹腔镜手术领域中可能特别有用。
Purpose. Postoperative intra-abdominal adhesion sometimes causes significant morbidity. The aim of this study was to compare the efficacy of our newly developed antiadhesive material, alginate flakes, to the most commonly used combination of hyaluronic acid and carboxymethyl cellulose film.Methods. Sodium alginate was formed into a gel, powder, or flakes. In the ex vivo study, these different alginate forms were attached onto pig skin and their antisolubility properties in saline and attachment stability were compared. In the in vivo study, a rat surgical adhesion model was used to study the properties of the alginates, and the rats were euthanized on day 14 after surgery. The efficacy of the antiadhesive materials was evaluated using an adhesion scoring system, and the locations that were treated with the antiadhesives were histologically examined.Results. In the alginate groups, the alginate flakes were superior with respect to the antisolubility and the attachment stability ex vivo as well as with respect to the antiadhesive efficacy in vivo. he adhesion score was almost the same as that observed in the alginate flake and cellulose film groups.Conclusions. We developed an alginate flake material and demonstrated its antiadhesive effects both ex vivo and in vivo. This is the first reported study using this flake-like material, which has a unique characteristic in that it can be applied by spraying in compressed air. Alginate flakes may therefore be especially useful in the field of laparoscopic surgery.