Identification of mutant monoclonal antibodies with increased antigen binding.
Identification of mutant monoclonal antibodies with increased antigen binding.
复制标题
鉴定抗原结合增加的突变单克隆抗体。
DOI：
10.1073/pnas.85.7.2298
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发表时间：
1988
影响因子：
11.1
通讯作者：
Scharff,MD
中科院分区：
文献类型：
--
作者：
Pollock,RR;French,DL;Gefter,ML;Scharff,MD
同胞选择和ELISA已用于分离产生突变抗体的杂交瘤亚克隆，所述突变抗体比亲本单克隆抗体更好地结合抗原。这种突变体在培养物中自发产生，频率为2.5-5 × 10 ~（-5）。突变体抗体的重链和轻链可变区的序列与亲本相同，并且突变体和亲本的Ka值相同。结合的增加与恒定区多肽的异常有关，可能反映了这些抗体亲合力的变化。
Sib selection and an ELISA have been used to isolate hybridoma subclones producing mutant antibodies that bind antigen better than the parental monoclonal antibody. Such mutants arise spontaneously in culture at frequencies of 2.5-5 X 10(-5). The sequences of the heavy and light chain variable regions of the mutant antibodies are identical to that of the parent and the Ka values of the mutants and the parent are the same. The increase in binding is associated with abnormalities of the constant region polypeptide and probably reflect changes in avidity of these antibodies.