Structure of the major neutral oligosaccharide-alditols released from the egg jelly coats of Axolotl maculatum. Characterization of the carbohydrate sequence GalNAc(beta 1-4)[Fuc(alpha 1-3)] GlcNAc(beta 1-3/6).
Structure of the major neutral oligosaccharide-alditols released from the egg jelly coats of Axolotl maculatum. Characterization of the carbohydrate sequence GalNAc(beta 1-4)[Fuc(alpha 1-3)] GlcNAc(beta 1-3/6).
复制标题
从蝾螈卵果冻层中释放的主要中性寡糖-糖醇的结构。
作者：
G. Strecker;J. Wieruszeski;M. Fontaine;Y. Plancke
用核磁共振波谱法分析了黄斑蝾螈卵周围胶壳上的几种o键低聚糖。通过还原-消除释放的四种主要低聚糖糖醇显示Lewisx （Lex）行列式或序列GalNAc(β 1-4)[Fuc(α 1-3)]GlcNAc。最后一种结构先前已在海鞘h抗原中分离的具有致敏活性的寡糖，以及曼氏血吸虫和人尿激酶的n -链聚糖中被表征。它代表了在A. maculatum中发现的主要碳水化合物链，其管道构成了β 1-4-乙酰半乳糖胺基转移酶活性的极好来源。此外，从黄斑竹中分离出的碳水化合物链与在其他七种两栖动物中发现的碳水化合物链有很大的不同，其中存在物种特异性物质。在受精过程中，碳水化合物的作用越来越明显，而o链低聚糖的多样性支持了这种生物学作用。
Several O-linked oligosaccharides of the jelly coat surrounding the eggs of Axolotl maculatum were analysed by 1H-NMR spectroscopy. The four major oligosaccharidealditols released by reductive beta-elimination display either the Lewisx (Lex) determinant or the sequence GalNAc(beta 1-4)[Fuc(alpha 1-3)]GlcNAc. This last structure has previously been characterized in allergenically active oligosaccharides isolated from the sea squirt H-antigen, and in the N-linked glycans of Schistosoma mansoni and human urokinase. It represents the major carbohydrate chain found in A. maculatum, the oviduct of which constitutes an excellent source of beta 1-4-acetylgalactosaminyl transferase activity. Moreover, the carbohydrate chains isolated from A. maculatum are quite different from those found in seven other amphibian species, in which the presence of species-specific material has been characterized. The role of carbohydrates appears more and more apparent during the fertilization process, and the diversity of the O-linked oligosaccharides supports such a biological role.