Head-tail connector of bacteriophage lambda.
Head-tail connector of bacteriophage lambda.
复制标题
λ噬菌体的头尾连接器。
DOI：
10.1016/0022-2836(80)90280-6
复制
发表时间：
1980
影响因子：
5.6
通讯作者：
Hendrix,RW
中科院分区：
文献类型：
--
作者：
Tsui,L;Hendrix,RW
噬菌体λ的头尾连接器是一个位于噬菌体头壳内的蛋白质球，其尾部与之相连，主要由头蛋白gpB§及其裂解形式gpB组成。病毒体中的所有gpB和gpB受体都位于连接体中。被认为在头部上形成与尾部结合的位点的蛋白质gpFII也位于连接子中。头蛋白gpE、gpD、X1和X2不是连接子的组分。这些鉴定是通过用盐酸胍破坏病毒粒子来完成的，以这种方式，头部和尾部分离，连接器连接到尾部，并确定哪些头部蛋白与尾部共同纯化。我们发现λE−感染的裂解物含有高比例的连接器连接的尾部。（头壳主要组件的基因编码。）连接器也存在于λ E-C-感染的尾部，认为gpE，gpC及其加工形式X1和X2对于生物活性连接器的组装都是不必要的。在E −和E −C−尾部的连接器中的gpB是未裂解的形式。在农杆菌E −宿主中，在λE−B−、λE−FII−或λE−感染的尾部未观察到连接子。
The head-tail connector of phage λ, a protein knob inside the head shell to which the tail attaches, is composed primarily of head protein gpB§and its cleaved form gpB∗. All of the gpB and gpB∗in the virion is located in the connector. gpFII, the protein that is thought to form the site on the head to which the tail binds, is also located in the connector. Head proteins gpE, gpD, X1 and X2 are not components of the connector. These assignments were made by disrupting virions with guanidine hydrochloride, in such a way that heads and tails separate with the connectors attached to the tails, and determining which head proteins co-purify with the tails.We find that lysates from aλE−infection contain a high proportion of tails with connectors attached. (GeneEcodes for the major component of the head shell.) Connectors are also present on tails from aλE−C−infection, arguing that gpE, gpC, and their processed forms, X1 and X2, are all unnecessary for assembly of biologically competent connectors. The gpB in the connectors onE−andE−C−tails is in the uncleaved form. Connectors are not seen on tails from infections byλE−B−,λE−FII−, orλE−in agroE−host.