Prolonged vaginal cornification and other changes in mice treated neonatally with coumestrol, a plant estrogen.
Prolonged vaginal cornification and other changes in mice treated neonatally with coumestrol, a plant estrogen.
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新生小鼠接受香豆雌酚（一种植物雌激素）治疗后，阴道角化时间延长，并发生其他变化。
DOI：
10.1080/15287398509530635
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Stokstad,EL
中科院分区：
文献类型：
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作者：
Burroughs,CD;Bern,HA;Stokstad,EL
本研究使用新生小鼠模型来确定雌性小鼠早期暴露于香豆雌酚（一种植物雌激素）是否会导致生殖道改变，类似于新生小鼠暴露于己烯雌酚（DES）后观察到的改变。新生雌性C57 BL/ Crgl小鼠在出生后的前5天每天皮下注射溶于0.005 ml二甲基亚砜（DMSO）的0.08 μg DES或100 μg香豆雌酚，或单独注射DMSO，或不接受治疗。根据已发表的子宫生物测定数据（使用年轻成年小鼠），选择的剂量在生物活性方面相当。每天观察两次，持续1.5个月，以确定眼睛和阴道完全张开的时间。一半的动物在40日龄时切除卵巢。阴道灌洗检查连续30天开始在2和5个月的年龄。DES和香豆雌酚显著提前了完全阴道开放的时间，并诱导了相当程度的卵巢非依赖性持续阴道角化。此外，香豆雌酚导致出血性卵泡的发生。
This study used the neonatal mouse model to determine if early exposure of female mice to coumestrol, a plant estrogen, would result in reproductive‐tract alterations similar to those seen after neonatal exposure to diethylstilbestrol (DES). Newborn female C57BL/ Crgl mice were given daily subcutaneous injections of 0.08 μg DES or 100 μg coumestrol in 0.005 ml dimethyl sulfoxide (DMSO), or DMSO alone, or were untreated, for the first 5 d of life. The doses chosen were equivalent in biological activity based on published uterine bioassay data (using young adult mice). Observations were made twice daily for 1.5 mo to determine the times of eye and complete vaginal opening. Half of the animals were ovariectomized at 40 d of age. Vaginal lavages were examined for 30 consecutive d beginning both at 2 and at 5 mo of age. DES and coumestrol significantly advanced the time of complete vaginal opening and induced a comparable degree of ovary‐independent persistent vaginal cornification. In addition, coumestrol resulted in the occurrence of hemorrhagic ovarian follicles.