Density of tumor-infiltrating granzyme B-positive cells predicts favorable prognosis in dogs with transitional cell carcinoma
Density of tumor-infiltrating granzyme B-positive cells predicts favorable prognosis in dogs with transitional cell carcinoma
复制标题
DOI：
10.1016/j.vetimm.2017.07.001
复制
发表时间：
2017-08-01
影响因子：
1.8
通讯作者：
Matsuki, Naoaki
中科院分区：
文献类型：
--
作者：
Inoue, Akiko;Maeda, Shingo;Matsuki, Naoaki
虽然肿瘤浸润淋巴细胞（TILs）在抗肿瘤免疫中起着关键作用，但它们在犬移行细胞癌（TCC）中的作用还没有得到充分的证明。本研究的目的是探讨TIL数量与膀胱TCC预后的关系。对32例犬膀胱移行细胞癌和10例正常膀胱组织进行了CD 3和颗粒酶B的免疫组化分析。犬TCC瘤周间质中CD 3（+）和颗粒酶B+细胞数量明显高于正常对照组。在TCC病例中，CD3（+）TIL数量与预后无显著相关性，而丰富的颗粒酶Ms与良好的预后相关。由于颗粒酶B+ TILs与肿瘤分期无关，因此颗粒酶TILs的存在可能是一个独立的预后因素。这些结果表明，颗粒酶TILs在抗肿瘤免疫中发挥作用，并抑制犬TCC中的肿瘤进展。
Although tumor-infiltrating lymphocytes (TILs) play a key role in anti-tumor immunity, their involvement in canine transitional cell carcinoma (TCC) is not well-documented. The objective of this study was to investigate the association between TIL number and prognosis in dogs with urinary bladder TCC. Immunohistochemical analysis of CD3 and granzyme B was performed using canine TCC (n = 32) and normal bladder (n = 10) tissues. The numbers of CD3(+) and granzyme B+ cells located in peritumoral stroma of canine TCC were significantly higher than those in normal controls. In TCC cases, the number of CD3(+) TILs was not significantly related to prognosis, whereas the abundant granzyme Ms were associated with favorable outcome. Since granzyme B+ TILs were not associated with the tumor stage, the presence of granzyme TILs may be an independent prognostic factor. These results suggest that granzyme TILs play a role in anti-tumor immunity and inhibit tumor progression in canine TCC.