DETERMINATION OF ACETYL AND FORMYL GROUPS BY GAS-LIQUID-CHROMATOGRAPHY
DETERMINATION OF ACETYL AND FORMYL GROUPS BY GAS-LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0021-9673(00)83064-2
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
DARBRE, A
中科院分区：
文献类型：
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作者：
CHAUHAN, J;DARBRE, A
描述了一种水解蛋白质、氨基酸和碳水化合物以释放N-和O-乙酰基和甲酰基的方法。用冠醚催化合成了乙酰苯甲酰甲基酯和甲酰基苯甲酰甲基酯，用火焰离子化检测器进行了气相色谱测定。给出了含有约20 nmol乙酰基或甲酰基的样品的定量结果。该方法也适用于N-和O-丙酰基的测定。
A method is described for the hydrolysis of proteins, amino acids and carbohydrates for the liberation of N- and O-acetyl and formyl groups. The acetyl and formyl phenacyl esters are prepared by means of crown ether catalysis and determined by GLC using the flame-ionization detector. Quantitative results are given with samples containing about 20 nmol of acetyl or formyl group. The method is also applicable to the determination of N- and O-propionyl groups.