Generation of a consensus sequence from prevalent and incident HIV-1 infections in West Africa to guide AIDS vaccine development
Generation of a consensus sequence from prevalent and incident HIV-1 infections in West Africa to guide AIDS vaccine development
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DOI：
10.1006/viro.2002.1577
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发表时间：
2002-10-10
期刊：
影响因子：
3.7
通讯作者：
Butera, S
中科院分区：
文献类型：
--
作者：
Ellenberger, DL;Li, B;Butera, S
我们考虑了几个关键问题，关于发展的DNA为基础的人类免疫缺陷病毒1型（HIV-1）疫苗：（1）候选疫苗的构建是来自事件或流行的HIV-1菌株;（2）是否应循环血浆病毒，存档的HIV-1前病毒回收外周血单核细胞，或两者都包括在内？为了解决这些问题，我们从居住在科特迪瓦阿比让的感染者中收集了流行的HIV-1毒株。从一组23株毒株中，22株为HIV-1 gag A亚型，其中19株与来自西非的重组HIV-1，CRF 02-AG毒株在遗传学上聚簇。通过对蛋白酶基因进行测序，证实了CRF 02-AG的嵌合基因组。产生共有gag p24蛋白序列，并且148个密码子中的147个与CRF 02-AG（lbNG）相同。无论序列来源（RNA、前病毒、偶发或流行）如何，gag p24共有序列高度代表了这些不同的病毒学区室。这些数据表明，由偶发和流行感染产生的共有序列可以为DNA疫苗提供适当的序列，并且在很大程度上代表了主要的循环病毒株。
We considered several key issues regarding the development of a DNA-based human immunodeficiency virus type 1 (HIV-1) vaccine: (1) should the candidate vaccine construct be derived from incident or prevalent HIV-1 strains; and (2) should circulating plasma virus, archived HIV-1 provirus recovered from peripheral blood mononuclear cells, or both be included? To address these questions, we collected circulating HIV-1 strains from infected individuals residing in Abidjan, Cote d'lvoire. From a panel of 23 strains, 22 were HIV-1 subtype A in gag, 19 of which phylogenetically clustered with the recombinant HIV-1, CRF02-AG strains from West Africa. The mosaic genome of CRF02-AG was confirmed by sequencing the protease gene. A consensus gag p24 protein sequence was generated and 147 of 148 codons were identical to CRF02-AG (lbNG). Regardless of the sequence origin (RNA, provirus, incident, or prevalent), the gag p24 consensus sequences were highly representative of these distinct virologic compartments. These data suggest that the consensus sequence generated from incident and prevalent infections may provide an appropriate sequence for a DNA vaccine and is largely representative of the major circulating viral strain.