Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
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高亲和力 IgM 单克隆抗体对乙型肝炎进行免疫诊断。
DOI：
10.1073/pnas.78.2.1214
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发表时间：
1981
影响因子：
11.1
通讯作者：
ZurawskiJr,VR
中科院分区：
文献类型：
--
作者：
Wands,JR;Carlson,RI;Schoemaker,H;Isselbacher,KJ;ZurawskiJr,VR
制备了针对乙型肝炎表面抗原（HBsAg）的高亲和单克隆IgG和IgM抗体，并用固相放射免疫法研究了它们的功能。125i标记的HBsAg结合研究表明，与常规制备的抗hbs试剂或其他高亲和力IgG单克隆抗hbs抗体相比，针对HBsAg的单克隆IgM抗体（抗hbs）偶联到固相载体上定量结合的HBsAg更多，速度更快。因此，IgM抗hbs也进行了放射标记，并开发了用于乙肝免疫诊断的IgM-IgM放射免疫测定法，该测定法的下限约为每ml血清HBsAg的100 pg +/- 30 （SEM）。与现有的商业放射测定法相比，初步研究表明，IgM-IgM测定法具有更高的敏感性，从而提高了对急性肝炎和输血后肝炎中hbsag相关决定因素的检测。单克隆IgM抗hbs和多决定因素HBsAg之间的多价相互作用可能是提高单克隆检测性能的重要因素。
High-affinity monoclonal IgG and IgM antibodies to hepatitis B surface antigen (HBsAg) have been prepared and their functional capabilities explored by means of solid-phase radioimmunoassays. 125I-labeled HBsAg binding studies indicated that monoclonal IgM antibodies against HBsAg (anti-HBs) coupled to a solid-phase support quantitatively bound more HbsAg at a faster rate than conventionally prepared anti-HBs reagents or other high-affinity IgG monoclonal anti-HBs antibodies. Consequently, IgM anti-HBs was also radiolabeled, and an IgM-IgM radioimmunoassay was developed for the immunodiagnosis of hepatitis B. The lower limit of this assay was approximately 100 pg +/- 30 (SEM) of HBsAg per ml of serum. Compared to available commercial radioassays, preliminary studies have shown the IgM-IgM assay to have increased sensitivity, which improved the detection of a HBsAg-associated determinant in acute hepatitis and post transfusion hepatitis. It is probable that the multivalent interaction between monoclonal IgM anti-HBs and the polydeterminant HBsAg is important in augmenting the performance of this monoclonal assay.