CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro
CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro
复制标题
CD24体外促进宫颈癌细胞增殖并抑制其凋亡
DOI：
10.3892/or.2015.4521
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发表时间：
2016-03-01
期刊：
影响因子：
4.2
通讯作者：
Zhou, Yanhong
中科院分区：
文献类型：
--
作者：
Pei, Zhen;Zhu, Guangchao;Zhou, Yanhong
蛋白质CD 24是一种细胞表面蛋白，似乎起粘附分子的作用;其表达已被证明与多种肿瘤的预后相关。本研究旨在探讨CD 24在宫颈癌中的作用及其机制。我们的研究结果表明，CD 24在宫颈癌组织中过表达与癌旁组织相比，通过qPCR，免疫组化和蛋白质印迹技术。为了探讨CD 24在宫颈癌中的作用机制，我们研究了CD 24对宫颈癌HeLa细胞增殖和凋亡的影响，发现CD 24过表达的HeLa细胞增殖明显增加。HeLa/CD 24细胞凋亡率较HeLa或HeLa/vector细胞明显降低。细胞凋亡与线粒体膜电位（DeltaPsim）降低、细胞内活性氧（ROS）和钙离子（Ca ~（2+））浓度升高密切相关。我们的结果表明，在宫颈癌HeLa细胞中过表达CD 24，导致Δ Psim增加，细胞内ROS和Ca 2+浓度降低。此外，我们发现，在体外宫颈癌组织中，CD 24与MAPK信号通路的失调相关。同时，我们发现CD 24过表达影响p38、JNK 2和c-Jun的表达。综上所述，我们的研究结果表明，CD 24在宫颈癌组织中上调，并通过影响宫颈癌中的MAPK信号通路发挥其功能。
The protein CD24 is a cell surface protein that appears to function as an adhesion molecule; its expression has been shown to correlate with prognosis in a variety of tumors. Herein, we investigated the possible role and mechanism of CD24 in cervical cancer. Our results showed that CD24 was overexpressed in cervical cancer tissues compared with that in the adjacent non-cancerous tissues by qPCR, immunohistochemistry and western blotting technologies. To explore the possible mechanism of CD24 in cervical cancer, we elucidated the effect of CD24 on the proliferation and apoptosis of cervical cancer HeLa cells and found that a considerable increase in cell proliferation was observed in the HeLa cells with CD24 overexpession. The rate of cell apoptosis was decreased in the HeLa/CD24 cells compared with the HeLa or HeLa/vector cells. Cell apoptosis is closely related with a reduction in mitochondrial membrane potential (Delta Psi m) and an increase in intracellular reactive oxygen species (ROS) and calcium ion (Ca2+) concentrations. Our results showed that overexpression of CD24 in the cervical cancer HeLa cells, led to an increase in Delta Psi m and a decrease in intracellular ROS and Ca2+ concentrations. Furthermore, we found that CD24 was correlated with dysregulation of the MAPK signaling pathway in cervical cancer tissues in vitro. At the same time, we found that CD24 overexpression affected the expression of p38, JNK2 and c-Jun in vitro. In summary, our results suggest that CD24 is upregulated in cervical cancer tissues and plays its functions by affecting the MAPK signaling pathway in cervical cancer.