Avian Mycoplasma identification using polymerase chain reaction amplicon and restriction fragment length polymorphism analysis
Avian Mycoplasma identification using polymerase chain reaction amplicon and restriction fragment length polymorphism analysis
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DOI：
10.2307/1592417
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发表时间：
1995-10-01
期刊：
影响因子：
1.4
通讯作者：
Johansen, D
中科院分区：
文献类型：
--
作者：
Lauerman, LH;Chilina, AR;Johansen, D
采用一般支原体聚合酶链反应（PCR）对9种禽支原体进行扩增。将扩增产物与24种限制性内切酶反应，对不同种类支原体的限制性内切片段长度多态性（RFLP）进行电泳分析。24个限制性内切酶中的4个（DraI、MseI、RsaI、Tsp509I）切断了所有9种支原体的PCR扩增子。利用PCR扩增子的RFLP分析，可以明显区分9种禽支原体。
A general mycoplasma polymerase chain reaction (PCR) was used to generate amplicon (DNA amplification product) from nine avian mycoplasma species. The PCR amplicons were reacted with 24 restriction enzymes, and the electrophoretic patterns of restriction fragment length polymorphism (RFLP) were evaluated for differences between species of mycoplasmas. Four (DraI, MseI, RsaI, Tsp509I) of the 24 restriction enzymes cut the PCR amplicon of all nine mycoplasma species. The nine avian mycoplasma species could be distinctly differentiated using the RFLP analysis of the PCR amplicon.