Demonstration of the presence of G-proteins in hepatic microsomal fraction.
Demonstration of the presence of G-proteins in hepatic microsomal fraction.
复制标题
证明肝微粒体部分中存在 G 蛋白。
DOI：
10.1016/0006-291x(88)90469-x
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发表时间：
1988
影响因子：
3.1
通讯作者：
Kraus-Friedmann,N
中科院分区：
文献类型：
--
作者：
Codina,J;Kimura,S;Kraus-Friedmann,N
分离的肝微粒体囊泡中的G蛋白的存在被证明。G蛋白通过其被霍乱和百日咳毒素ADP-核糖基化的能力来鉴定。霍乱毒素鉴定出42和45 kDa蛋白，分别对应于αs-1和αs-2。百日咳毒素鉴定了一个40 kDa的蛋白质，对应于αi。微粒体G蛋白与质膜上相应的G蛋白相同，但比例不同;微粒体中的α s蛋白比质膜上的少得多。
The presence of G-proteins in isolated hepatic microsomal vesicles is demonstrated. The G-proteins were identified by their capacity to be ADP-ribosylated by cholera and pertussis toxins. Cholera toxin identified 42 and 45 kDa proteins, corresponding toαs-1 andαs-2, respectively. Pertussis toxin identified a 40 kDa protein corresponding toαi. The microsomal G-proteins are identical to the corresponding G proteins of the plasma membrane, but are present in different proportions; the microsomes have considerably lessαsproteins than the plasma membrane.