Fine-structure molecular typing of Irish Helicobacter pylori isolates and their genetic relatedness to strains from four different continents
Fine-structure molecular typing of Irish Helicobacter pylori isolates and their genetic relatedness to strains from four different continents
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DOI：
10.1128/jcm.41.12.5755-5759.2003
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发表时间：
2003-12-01
影响因子：
9.4
通讯作者：
Smyth, CJ
中科院分区：
文献类型：
--
作者：
Carroll, IM;Ahmed, N;Smyth, CJ
对74株爱尔兰幽门螺杆菌菌株进行了4个不同基因座(VacA信号序列和中间区、CAG致病岛3‘端插入-缺失多态和cagA)的基因分型。主要的VacA等位基因和插入-缺失基序表明爱尔兰分离株与特定的东亚或北欧株之间存在祖先关系。此外，对来自4个不同大洲的32株爱尔兰幽门螺杆菌代表菌株和22株爱尔兰幽门螺杆菌代表菌株进行了荧光扩增片段长度多态性基因分型和系统发育分析，结果表明，所检测的爱尔兰幽门螺杆菌菌株均为弱克隆株，且与欧洲和亚洲分离株均有一定的相关性。这三种基因分型技术表明，爱尔兰幽门螺杆菌分离株具有独特的特征，这些特征可能是在这个与世隔绝的欧洲人口中进化出来的。
Genotyping of 74 Irish Helicobacter pylori isolates was performed at four different loci (vacA signal sequence and mid-region, insertion-deletion polymorphisms at the 3' end of the cag pathogenicity island, and cagA). The predominant vacA alleles and insertion-deletion motifs suggest an ancestral relationship between Irish isolates and either specific East Asian or Northern European strains. In addition, fluorescent amplified fragment length polymorphism-PCR genotyping and phylogenetic analysis of 32 representative Irish H. pylori isolates and 22 isolates from four different continents demonstrated that the Irish H. pylori isolates examined were weakly clonal and showed some association with both European and Asian isolates. These three genotyping techniques show that Irish H. pylori isolates have distinctive features that may have evolved in this insular European population.