TRM61 is essential for Arabidopsis embryo and endosperm development
TRM61 is essential for Arabidopsis embryo and endosperm development
复制标题
TRM61 对于拟南芥胚胎和胚乳发育至关重要
DOI：
10.1007/s00497-021-00428-x
复制
发表时间：
2021-08-18
影响因子：
3.4
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
--
作者：
Aslam, Mohammad;Huang, Xiaoyi;Qin, Yuan
TRNA分子的转录后修饰在基因表达和蛋白质生物合成中起着至关重要的作用。在整个属中，AtTRM61/AtTRM6复合体对腺苷58(A58)N1位tRNAs的甲基化在维持启动子甲硫酰tRNA(TRNAiMet)的稳定性中起着至关重要的作用。最近的研究表明，AtTRM61或AtTRM6的突变会导致种子败育。然而，关于AtTRM61/AtTRM6在植物中的功能的详细研究仍然很模糊。在这里，我们发现AtTRM61具有保守的功能结构和与辅因子S-腺苷-L-蛋氨酸(ADOMet)保守的结合基序。AtTRM61/AtTRM6复合亚单位突变会导致胚胎和胚乳发育缺陷。Attrm61-1/+FIS2PRO：：AtTRM61和Attrm61-1/+ABI3PRO：：AtTRM61有条件地补充了胚乳和胚乳的发育缺陷，表明AtTRM61是早期胚胎和胚乳发育所必需的。此外，通过过表达启动子tRNA来挽救trm61/+中的生育缺陷，表明AtTRM61突变可能会降低tRNAi的Met稳定性。此外，通过酵母双杂交(Y2H)和双分子荧光互补(BIFC)实验，我们证明了AtMPK4与AtTRM61在物理上相互作用。这些数据表明AtTRM61在拟南芥中具有保守的结构和功能。此外，AtTRM61可能是tRNAi代谢稳定性、胚和胚乳发育所必需的。
Post-transcriptional modifications of tRNA molecules play crucial roles in gene expression and protein biosynthesis. Across the genera, methylation of tRNAs at N1of adenosine 58 (A58) by AtTRM61/AtTRM6 complex plays a critical role in maintaining the stability of initiator methionyl-tRNA (tRNAiMet). Recently, it was shown that mutation in AtTRM61 or AtTRM6 leads to seed abortion. However, a detailed study about the AtTRM61/AtTRM6 function in plants remains vague. Here, we found that AtTRM61 has a conserved functional structure and possesses conserved binding motifs for cofactor S-adenosyl-L-methionine (AdoMet). Mutations of the complex subunits AtTRM61/AtTRM6 result in embryo and endosperm developmental defects. The endosperm and embryo developmental defects were conditionally complemented by Attrm61-1/ + FIS2pro::AtTRM61 and Attrm61-1/ + ABI3pro::AtTRM61 indicating that AtTRM61 is required for early embryo and endosperm development. Besides, the rescue of the fertility defects in trm61/ + by overexpression of initiator tRNA suggests that AtTRM61 mutation could diminish tRNAiMetstability. Moreover, using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays, we showed that AtMPK4 physically interacts with AtTRM61. The data presented here suggest that AtTRM61 has a conserved structure and function in Arabidopsis. Also, AtTRM61 may be required for tRNAiMetstability, embryo and endosperm development.