Estradiol metabolism in isolated human endometrial epithelial glands and stromal cells.
Estradiol metabolism in isolated human endometrial epithelial glands and stromal cells.
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分离的人子宫内膜上皮腺和基质细胞中的雌二醇代谢。
DOI：
10.1210/endo-104-6-1674
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发表时间：
1979
期刊：
影响因子：
4.8
通讯作者：
L. Tseng
中科院分区：
文献类型：
--
作者：
H. Liu;L. Tseng
用0.1%胶原酶消化法从正常增殖期和分泌期子宫内膜组织中分离出完整的上皮腺和间质细胞。这些成分是通过腺体的结构和细胞培养的独特形态来鉴定的。在来源于腺体或基质的原代细胞培养中，其形态特征和雌二醇脱氢酶(E2DH)活性一代代保持不变。培养的间质细胞的生成时间比腺上皮细胞短。在培养液中添加雌二醇(E_2)和乙酸甲羟孕酮可加快两种细胞的生长速度。在新鲜分离的腺体和基质细胞中，通过以不同的浓度和时间间隔与放射性的雌二醇组分或雌酮(E1)孵育来研究雌二醇代谢。雌二醇代谢模式的质的和量的变化在附图上有明显的区别。
Intact epithelial glands and stromal cells were isolated from normal proliferative and secretory endometrial specimens by digesting the tissue fragments with 0.1% collagenase solution. These components were identified by the structure of the glands and the distinct morphology of the cell cultures. The same morphological characteristics and estradiol dehydrogenase (E2DH) activity were maintained from generation to generation in the primary cell cultures derived from either glands or stroma. The generation time of cultured stromal cells was shorter than that of glandular epithelial cells. The growth rate of both cell cultures was accelerated by addition of estradiol (E2) and medroxyprogesterone acetate in culture medium. Metabolism of E2 was studied in the freshly isolated glands and stromal cells by incubating these components, with radioactive E2 or estrone (E1) at various concentrations and time intervals. Qualitative and quantitative variations in the metabolic pattern of E2 were distinguished in epithe...