The impact of intracellular aminopeptidase on C2C12 myoblast proliferation and differentiation
The impact of intracellular aminopeptidase on C2C12 myoblast proliferation and differentiation
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DOI：
10.1016/j.bbrc.2020.01.115
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发表时间：
2020-04-09
影响因子：
3.1
通讯作者：
Nagatomi, Ryoichi
中科院分区：
文献类型：
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作者：
Osana, Shion;Murayama, Kazutaka;Nagatomi, Ryoichi
泛素-蛋白酶体途径对骨骼肌的生长和发育至关重要。蛋白酶体在细胞质中产生寡肽，并且这些肽被认为被几种细胞内氨基肽酶快速降解为氨基酸。然而，细胞内氨基肽酶在肌肉生长中的作用仍然未知。因此，在本研究中，我们研究了细胞内氨基肽酶在C2 C12成肌细胞增殖和分化中的作用。抑制细胞内氨基肽酶的Bestatin甲酯（Bes-ME）降低亮氨酸和丙氨酸氨基肽酶的活性，并损害C2 C12成肌细胞的增殖和分化。此外，我们观察到细胞内氨基肽酶的抑制降低了细胞内氨基酸水平和ATP水平，并抑制了mTOR通路的磷酸化。这些结果表明，细胞内氨基肽酶通过mTOR途径影响C2 C12成肌细胞的增殖和分化;然而，需要进一步的研究来阐明氨基肽酶在骨骼肌中的作用。(C)2020年，任作家。爱思唯尔公司出版
The ubiquitin-proteasome pathway is essential for skeletal muscle growth and development. Proteasomes generate oligopeptides in the cytoplasm, and these peptides are considered to be rapidly degraded to amino acids by several intracellular aminopeptidases. However, the role of intracellular aminopeptidases in muscle growth remains unknown. In this study, therefore, we investigated the role of intracellular aminopeptidases in C2C12 myoblast proliferation and differentiation. Inhibition of intracellular aminopeptidases by Bestatin methyl ester (Bes-ME) decreased leucine and alanine aminopeptidase activity, and impaired proliferation and differentiation of C2C12 myoblasts. Furthermore, we observed that the inhibition of intracellular aminopeptidases reduced intracellular levels of amino acid and ATP level, and suppressed the phosphorylation of the mTOR pathway. These results suggested that intracellular aminopeptidases affect C2C12 myoblast proliferation and differentiation via mTOR pathway; however, further studies are required to clarify the role of aminopeptidase in skeletal muscle. (C) 2020 The Authors. Published by Elsevier Inc.