SULPHATE ((SO4)-S-35) UPTAKE BY CHONDROCYTES IN RELATION TO HISTOLOGICAL CHANGES IN OSTEOARTHRITIC HUMAN ARTICULAR CARTILAGE
SULPHATE ((SO4)-S-35) UPTAKE BY CHONDROCYTES IN RELATION TO HISTOLOGICAL CHANGES IN OSTEOARTHRITIC HUMAN ARTICULAR CARTILAGE
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DOI：
10.1136/ard.19.4.318
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发表时间：
1960-01-01
影响因子：
27.4
通讯作者：
MCELLIGOTT, TF
中科院分区：
文献类型：
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作者：
COLLINS, DH;MCELLIGOTT, TF
对人体软骨细胞对硫酸盐摄取的定量研究（McElligott and Collins, 1960）表明，随着年龄的增长，关节软骨的软骨细胞活性增加，而肋软骨的软骨细胞活性则下降。在有关节软骨破坏和纤颤的裸眼证据的情况下，记录了相当增强的关节软骨细胞活动。我们假设在老化的关节软骨中硫酸盐利用率的增加、骨关节炎的程度以及已知在这些情况下发生的软骨细胞的特殊重排和分组之间可能存在关系。为了研究这一点，用标记硫酸盐孵育的软骨的大量放射自显影片已经被制备和研究，并与用各种方法染色的平行切片并排研究，包括那些显示粘多糖的切片。我们现在报告的正是这些研究的结果。材料与方法34例4 ~ 83岁患者的尸体解剖中，每例取髌骨。标本在取出后尽快处理，所有病例均在2小时内处理。在这段时间内，标本被保存在40℃的培养培养基中，这一过程可以降低软骨细胞活性的损失率。死亡和尸检之间以及标本收集和处理之间的间隔都被仔细记录下来。
Quantitative studies of sulphate uptake by the cells of human cartilage (McElligott and Collins, 1960) have shown that chondrocyte activity increases in articular cartilage as age advances, while it decreases in costal cartilage. A considerably enhanced activity of articular over costal cartilage cells was recorded in cases where there was nakedeye evidence of destruction and fibrillation of the articular cartilage. We postulated a possible relationship between the increased sulphate utilization of ageing articular cartilage, the degree of osteo-arthritis, and the peculiar rearrangement and grouping of chondrocytes that are known to occur in these conditions. In order to investigate this, a large number of autoradiographs of cartilage incubated with labelled sulphate have been prepared and studied side by side with parallel sections stained by various methods including those that demonstrate mucopolysaccharides. It is the results of these studies that we now report.Material and Methods A patella was removed at each of 34 necropsies from persons ranging in age from 4 to 83 years. Specimens were processed as soon as possible after removal and in all cases within 2 hours. During this delay the specimens were stored at 40 C. in the incubation medium, a pro-cedure that reduces the rate of loss of chondrocyte activity. The intervals between death and autopsy and between the collection of the specimen and its processing were carefully noted.