Epifluorescence collection in two-photon microscopy
Epifluorescence collection in two-photon microscopy
复制标题
DOI：
10.1364/ao.41.005376
复制
发表时间：
2002-09-01
期刊：
影响因子：
1.9
通讯作者：
Mertz, J
中科院分区：
文献类型：
--
作者：
Beaurepaire, E;Mertz, J
我们提出了一个简单的模型来描述双光子显微镜中的落射荧光收集，当用物镜在浑浊的平板上拍摄图像时。体散射和表面散射决定了板表面出射荧光光子的空间和角度分布，几何光学决定了收集光子的效率。收集光学器件通过物镜的数值孔径和工作距离以及有效收集视场进行参数化。我们确定了每个参数的作用，并为优化落射荧光收集效率提供了简单的经验法则。蒙特卡罗模拟验证了分析结果。 (C) 2002 年美国光学学会。
We present a simple model to describe epifluorescence collection in two-photon microscopy when one images in a turbid slab with an objective. Bulk and surface scattering determine the spatial and angular distributions of the outgoing fluorescence photons at the slab surface, and geometrical optics determines how efficiently the photons are collected. The collection optics are parameterized by the objective's numerical aperture and working distance and by an effective collection field of view. We identify the roles of each of these parameters and provide simple rules of thumb for the optimization of the epifluorescence collection efficiency. Analytical results are corroborated by Monte Carlo simulation. (C) 2002 Optical Society of America.