Antisense RNA directed against the 3' noncoding region prevents dormant mRNA activation in mouse oocytes.
Antisense RNA directed against the 3' noncoding region prevents dormant mRNA activation in mouse oocytes.
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针对 3 非编码区的反义 RNA 可防止小鼠卵母细胞中休眠的 mRNA 激活。
DOI：
10.1126/science.2456615
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Vassalli,JD
中科院分区：
文献类型：
--
作者：
Strickland,S;Huarte,J;Belin,D;Vassalli,A;Rickles,RJ;Vassalli,JD
原代小鼠卵母细胞含有组织纤溶酶原激活物（t-PA）的非翻译稳定信使RNA。在减数分裂成熟过程中，这种母体mRNA经历3′-聚腺苷酸化，被翻译并降解。注射不同的反义RNA互补的t-PA mRNA的编码和非编码部分的成熟卵母细胞都选择性地阻断t-PA的合成。注射成熟卵母细胞后，t-PA mRNA的RNA印迹分析表明，RNA → RNA杂合区发生裂解，产生稳定的5′端部分和不稳定的3′端部分。在原代卵母细胞中，3′端非编码区易被切割，而mRNA的其他部分在成熟前被阻止形成杂交体。注射与t-PA末端3′非翻译区103个核苷酸互补的反义RNA足以阻止t-PA mRNA的多聚腺苷酸化、翻译激活和不稳定。这些结果表明，在小鼠卵母细胞减数分裂成熟过程中，休眠mRNA的3′非编码区在其翻译募集中起着关键作用。
Primary mouse oocytes contain untranslated stable messenger RNA for tissue plasminogen activator (t-PA). During meiotic maturation, this maternal mRNA undergoes a 3′-polyadenylation, is translated, and is degraded. Injections of maturing oocytes with different antisense RNA's complementary to both coding and noncoding portions of t-PA mRNA all selectively blocked t-PA synthesis. RNA blot analysis of t-PA mRNA in injected, matured oocytes suggested a cleavage of the RNA⋅RNA hybrid region, yielding a stable 5′ portion, and an unstable 3′ portion. In primary oocytes, the 3′ noncoding region was susceptible to cleavage, while the other portions of the mRNA were blocked from hybrid formation until maturation occurred. Injection of antisense RNA complementary to 103 nucleotides of its extreme 3′ untranslated region was sufficient to prevent the polyadenylation, translational activation, and destabilization of t-PA mRNA. These results demonstrate a critical role for the 3′ noncoding region of a dormant mRNA in its translational recruitment during meiotic maturation of mouse oocytes.